| ABSTRACT | 第1-6页 |
| 摘要 | 第6-13页 |
| CHAPTER ONE LITTERATURE REVIEW | 第13-35页 |
| ·Impact of abiotic stress in plants | 第13-14页 |
| ·Methods Developed in the Control of Plants Abiotic Stress | 第14-24页 |
| ·Maintenance of Soil Biodiversity | 第14-15页 |
| ·Positive Interactions between Plant Species | 第15-16页 |
| ·C onventional Breeding to Genetic Engineering | 第16-24页 |
| ·Harmfull effect of salinity in plants: Impact and metabolisim | 第24-25页 |
| ·Saline soil and agricultural yields | 第24-25页 |
| ·Effect of salinity in plant metabolism | 第25页 |
| ·DREB2A response abiotic stress and mechanism | 第25-31页 |
| ·Dehydration Response Element Binding factors and salinity stress | 第26-28页 |
| ·DREB mechanism involved in stress response | 第28-31页 |
| ·Progress in breeding of Rosa chinensis Jack. | 第31-35页 |
| ·General objectives | 第33页 |
| ·Specific objectives | 第33-35页 |
| CHAPTER TWO EVALUATING SALT TOLERANCE ABILITY OF TISSUE CULTURE AND GREENHOUSE SCREENING OF IMPROOVED ROSA CHINENSIS JACQ | 第35-76页 |
| ·EXPERIMENTAL MATERIALS | 第35-41页 |
| ·Plant material | 第35-36页 |
| ·Experimental equipments | 第36页 |
| ·Important reagents | 第36-38页 |
| ·Tissue culture medium | 第38-41页 |
| ·METHODOLOGY | 第41-46页 |
| ·Plant Material | 第41页 |
| ·Culture media and conditions | 第41-42页 |
| ·Total Chlorophyl and Proline determination (Min et al. 2009;Monreal et al. 2007) | 第42-43页 |
| ·Measurement of Potassium (K), Nitrogen (N), Phosphorus (P) | 第43页 |
| ·Measurement of soil water, salt and pH values | 第43-44页 |
| ·Screening for selection of resistant plants with stimulation at the branch burgeon point (BBP) | 第44-45页 |
| ·Sample preparation for Transmission Electron Microscope (TEM) study | 第45页 |
| ·Statistical analysis | 第45-46页 |
| ·RESULTS | 第46-66页 |
| ·Efficiency of medium composition on embryo development,proliferation and differenciation under NaCl stress conditions. | 第46-48页 |
| ·Physical characteristics of tissue development and influence of NaCl stress on Shoots regeneration | 第48-52页 |
| ·Physiological analysis of control, SSIV and RIV plants | 第52-54页 |
| ·Rose improved cell engineering system and physiological and ultrastructural analysis of control and bred variety | 第54-66页 |
| ·DISCUSSIONS | 第66-74页 |
| ·Hormones concentrations can provide a resistance effect to NaCl stress Rosa chinensis Jacq. tissue regeneration | 第66-67页 |
| ·Plantlets recovery and regeneration require a prolific NaCl stress aleviation | 第67-68页 |
| ·NaCl stress regenerated plants were improved of proline and water content | 第68-69页 |
| ·Bred plants NaCl stress and metabolites regulation | 第69-71页 |
| ·Bred plants NaCl stress and leaf ultrastructure analysis | 第71-74页 |
| ·SUMMARY | 第74-76页 |
| CHAPTER THREE EXPRESSI ON OF AtDREB2A-CA TO ARABIDOPSIS THALIANA REVEALS NACL TOLERANCE | 第76-113页 |
| ·EXPERIMENTAL MATERIAL | 第76-79页 |
| ·Agrobacterium strain, primers and plant material | 第76页 |
| ·Important apparatus | 第76-77页 |
| ·Important reagents | 第77-78页 |
| ·Culture medium | 第78-79页 |
| ·Important solutions | 第79页 |
| ·METHODOLOGY | 第79-89页 |
| ·Confirmation of AtDREB2A-CA expression gene in C58 Agrobacterium tumefaciens strain | 第79-80页 |
| ·Plasmid extraction method | 第80页 |
| ·PCR analysis and agarose gel electrophoresis | 第80-81页 |
| ·Arabidopsis thaliana transformation method | 第81-86页 |
| ·Identification of transgenic line | 第86-88页 |
| ·Transgenic arabidopsis analysis of salt tolerance –plant physiology and ultrastructure | 第88-89页 |
| ·In vitro analysis of plants root elongation under H202, LiCl, and NaCl treatment | 第89页 |
| ·Statistical analysis | 第89页 |
| ·RESULTS | 第89-108页 |
| ·Agrobacterium tumefaciens C58 expressing the vector pH7m24GW,31c-prd29A-AtDREB2ACA and PCR detection of the AtDREB2A-CA gene | 第89-91页 |
| ·Detection of genetically modified plants | 第91-94页 |
| ·NaCl tolerance analysis in greenhouse transgenic A. thaliana | 第94-105页 |
| ·Germination and Root development of in vitro WT and AtDREB2A-CA transgenic A. thaliana cultured in H_20_2 and LiClsupplemented media | 第105-108页 |
| ·DISCUSSION | 第108-111页 |
| ·AtDREB2A-CA transgenic Arabidopsis thaliana and physiological response to NaCl stress | 第108-109页 |
| ·AtDREB2A-CA expressing Arabidopsis thaliana and leaf ultrastructure in response to NaCl stress | 第109-110页 |
| ·H_20_2 and LiCl - induced Germination and leaf revealed by AtDREB2A-CA expression in Arabidopsis thaliana | 第110-111页 |
| ·SUMMARY | 第111-113页 |
| CHAPTER FOUR AtDREB2A-CA GENE EXPRESSION AND APPLICATION FOR SALINITY STRESS RESISTANCE TO ROSA CHINENSIS JACQ | 第113-145页 |
| ·EXPERIMENTAL MATERIAL | 第113-117页 |
| ·Agrobacterium strain, primers and plant material | 第113-114页 |
| ·Important apparatus | 第114页 |
| ·Important reagents | 第114-115页 |
| ·Culture medium | 第115页 |
| ·Important solutions | 第115-117页 |
| ·METHODOLOGY | 第117-123页 |
| ·Agrobacterium tumefaciens mediated Rosa chinensis Jacq. transformation method | 第117-119页 |
| ·Identification of transgenic line | 第119-120页 |
| ·Transgenic Rosa chinensis Jacq. Physiological activity and an alysis of the effect of NaCl on plant ultrastructure | 第120页 |
| ·Western blot analysis | 第120-122页 |
| ·Statistical analysis | 第122-123页 |
| ·Results | 第123-139页 |
| ·BBPs in Rosa chinensis Jacq. Ag robacterium tumefaciens mediated transformation and regeneration | 第123-128页 |
| ·PCR and RT-PCR amplification analysis | 第128-129页 |
| ·Water, Cha+b, Total carotenoids, pr oline analysis, Nitrogen, Phosphorus and Potassium analysis | 第129页 |
| ·Western Blot of SOD protein | 第129-130页 |
| ·Rosa chinensis Jacq. leaf ultrastructure in response to NaCl stress. | 第130-139页 |
| ·DISCUSSION | 第139-143页 |
| ·The ideal BBP and conditions for Rosa chinensis Jacq. Agrobacterium tumefaciens mediated transformation and regeneration | 第139-142页 |
| ·Rosa chinensis Jacq. expressing AtDREB2A-CA plant physiological activity and NaCl tolerance | 第142页 |
| ·Leave ultrastructure modulated in response to AtDREB2A-CA expression confering a transgenic Rosa chinensis Jacq NaCl stress tolerant | 第142-143页 |
| ·Summary | 第143-145页 |
| CHAPTER FIVE CONCLUSION AND PERSPECTIVES | 第145-149页 |
| ·CONCLUSION | 第145-146页 |
| ·INNOVATION | 第146-147页 |
| ·PERSPECTIVES | 第147-149页 |
| REFERENCES | 第149-166页 |
| LIST OF ABBREVIATIONS | 第166-168页 |
| LIST OF FIGURES | 第168-171页 |
| LIST OF TABLES | 第171-172页 |
| STATUS OF PAPER PUBLICATION AND CONFERENCE ATTENDED | 第172-174页 |
| PUBLISHED PAPERS | 第172页 |
| PUBLICATIONS IN PREPERATION | 第172-173页 |
| CONFERENCE ATTENDED | 第173-174页 |
| ACKNOWLEDGEMENTS | 第174-175页 |
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