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血链球菌部分基因克隆及与nif基因同源序列初探
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【中医学评职论文下载】潘亚萍 王红杨 金春元 孙开来摘要 目的:分析血链球菌染色体DNA片段序列,探讨血链球菌遗传生物学特性。方法:采用TA克隆技术将此片段转化克隆并进行碱基序列测定,通过GenBank进行同源分析。结果:血链球菌ATCC10556 DNA片段含有与固氮菌高度同源的nifS、nifU基因,同源分值为114点(bits)。结论:国内外首次报道血链球菌DNA片段含有nifS、nifU部分基因。关键词 血链球菌 基因克隆 nif基因
Partial Gene Clone and nif Gene Homologous Sequence Analysisof Streptococcus sanguis
Pan Yaping, Wang HongyangDepartment of Oral Medicine, Dental School, China Medical UniversityJin Chunyuan, Sun KailaiDepartment of Heredity, Basic Medicine College, China Medical University
Abstract Objective: To analyze the sequence of Streptococcus sanguis chromosome which contains one DNA fragment of 800 base pairs (bp) and discuss Streptococcus sanguis biological features of heredity. Methods: Streptococcus DNA of 800-bp genetic fragment was cloned and analyzed by using eukaryotic expression vector. Results:By Genebank's database, it showed that the 800-bp genetic
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