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HPLC法测定人唾液中卡马西平浓度及其药代动力学
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【医学论文代发】 作者：唐哲，文爱东，吴寅，赵磊，李宏力，石茹【关键词】 卡马西平Determination of carbamazepine concentration in human saliva by HPLC and its pharmacokinetic study
【Abstract】 AIM: To determine carbamazepine(CBZ) concentration in human saliva by HPLC and study its pharmacokinetics. METHODS: Shimpack CLCODS C18 (150 mm×6 mm, 5 μm) spectrum analytical column was used and methanolwater(53∶47)was adopted as the mobile phase. Flow rate was 1.0 mL・min-1, detection wavelength was 212 nm, column temperature was 23℃ and the dosage of CBZ was 50 μL. The pharmacokinetic parameters of CBZ tablets taken by 6 healthy volunteers at a single oral dose of 200 mg were calculated according to CBZ concentration in saliva. RESULTS: The standard curve was linear (r=0.999) within the range of 0.40~4.32 mg・L-1 for CBZ. The limit of detection in saliva was 30 μg・L-1 (S/N≥3). The ave
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