| | | 中药肝复健冲剂对大鼠肝纤维化形成的影响
| | 论文下载【关键词】 胶原
Influence of Ganfujian granule formula on process of liver fibrosis in rat
【Abstract】 AIM: To investigate the effect of ganfujian granule on collagen sediment in rat hepatic fibrosis induced by diethylnitrosamine (DEN). METHODS: DEN induced live fibrosis model was established and ganfujian (GFJ) granule was administrated as intervention measure. Influence (EFJ group) of ganfujian granule on collagen sediment and hepatocyte proliferation were examined by Picric acidSirus red staining and immunohistochemical staing methods, and analyzed by computer image analysis technique. RESULTS: Collagen sediment in rat liver increased gradually with the exacerbation of hepatic fibrosis in the model. Inflammatory damage and the degree of liver cirrhosis were lower GFJ group than in model group. Collagen sediment in liver and positive expression of proliferotive cell number ontgen (PCNA) in hepatocytes were decreased significantly in GFJ group (collagen sediment: 4 wk （15.20±2.21）vs（7.19±0.97）;12 wk （27.1±4.6）vs （16.0±1.7）. positive expression of PCNA in hepatocytes: 12 wk （21.1±0.32） vs （6.4±0.2）; 16 wk（31.1±7.1）vs（10.1±0.8） (P<0.05). CONCLUSION: GFJ granule can inhibit and retard rats liver fibrosis by reduce collagen sediment in rat liver and may prevent liver cirrhosis transit to hepatocarcinoma in some degree by inhibit overproliferating of hepatocytes.
【Keywords】ganfujian granule; traditional medicine; liver cirrhosis; collagen
【摘要】 目的论文下载： 观测中药肝复健冲剂对二乙基亚硝胺（DEN）诱导大鼠肝纤维化形成的论文下载影响. 方法： 建立DEN诱导大鼠肝硬化模型，同时给予肝复健冲剂进行干预，应用天狼红苦味酸特染法、免疫组化染色法及计算机图像分析技术检测中药对DEN致大鼠肝纤维化过程胶原沉淀及肝细胞增殖特性的影响. 结果： 随着模型大鼠肝纤维化程度加重，肝内胶原沉积量亦呈逐渐增加趋势. 中药组大鼠肝脏炎性损害及肝纤维化程度均较同期模型组大鼠轻，胶原沉积量及细胞增殖核抗原(PCNA)阳性着色肝细胞数明显减少，两组间差异有统计学意义, P<0.05［胶原沉积量：4 wk（15.20±2.21）vs （7.19±0.97）; 12 wk（27.1±4.6） vs （16.0±1.7）. 阳性表达PCNA肝细胞数：12 wk（21.1±0.32） vs （6.4±0.2）;16 wk（31.1±7.1）vs （10.1±0.8）］. 结论： 中药肝复健冲剂可通过减少肝内胶原沉积及可能恶性增殖的肝细胞，抑制和延缓实验性动物肝纤维化的形成，并一定程度上预防肝硬化向肝癌的转变.
【关键词】 肝复健冲剂；肝硬化；胶原
0引言
HBV感染患者和几乎全部HCV携带者有可能发展为肝硬化，亦是造成肝硬化发病率居高不下的主要原因，而肝硬化中约30%患者可并发肝癌［1］. AFP升高、肝硬化伴细胞不典型增生或细胞增殖活性增高成为肝癌发病的高危因素［2］，因而慢性肝病、肝硬化的防治十分重要. 如何延缓肝纤维化形成进程或逆转至正常，已成为肝硬化及肝癌防治的中心问题. 因此，研制有效且可长期服用的预防制剂也越来越引起人们的关注. 健脾扶正法是中医临床防治肝硬化与肝癌常用的方法之一［3］. 本科室根据中医基础理论及临床实践研制的以健脾益气立法，由淮山药、山楂、大枣等药食同源中药组成的肝复健冲剂，在临床肝硬化防治过程中取得较好疗效.
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