| | | 白藜芦醇诱导肿瘤细胞凋亡和细胞周期阻滞的作用及机制
| | 教育论文下载 作者：朱青，张王刚，王立锋，王芳，杨安钢
【关键词】 白藜芦醇
Resveratrol induced apoptosis and cell arrest in cancer cells
【Abstract】 AIM: To study the mechanism of resveratrol induced HepG2 and K562 cells apoptosis and cell arrest. METHODS: Cell morphological method and Annexin V technology were used to detect HepG2 and K562 cells apoptosis, flow cytometry (FCM) was used to detect the cell cycle and MTT was used to detect the medicine sensitivity of HepG2 and K562 cells. RESULTS: Apoptosis was seen in human liver cancer HepG2 cell treated with resveratrol and nuclear chromatine condensation and fragmentation were observed in HepG2 cells. Typical ladder patterns of DNA fragmentation were also observed. The highest rate of HepG2 cell apoptosis was 33.7% by Annexin V. Cell cycle stopped at S phase and cell apoptosis rate was 9.97% by FCM. Time and dose dependent effects of resveratrol to HepG2 cell were detected by MTT. Resveratrol had no effect on K562 cells. CONCLUSION: Resveratrol induces HepG2 cell apoptosis and inhibits the development of human liver cancer with a time and dose dependent effect. The resveratrolinduced cell apoptosis is specific to cancer cells.
【Keywords】 resveratrol; apoptosis; cell cycle; hepG2; K562
【摘要】 目的教育论文下载: 探讨白藜芦醇(RES)诱导人肝癌HepG2、人慢粒K562肿瘤细胞凋亡和细胞周期阻滞的教育论文下载作用及机制. 方法: 应用形态学方法,Annexin V荧光染色检测HepG2，K562细胞凋亡的发生,应用流式细胞术(FCM)检测细胞周期，应用四唑蓝比色法(MTT)检测HepG2，K562肿瘤细胞对,RES的药物敏感性. 结果: RES对人肝癌HepG2细胞的生长有明显的抑制作用,并诱导肿瘤细胞发生凋亡. 凋亡细胞表现为细胞固缩, 核染色质碎裂. Annexin V荧光染色检测HepG2细胞凋亡率为33.7%,用FCM检测细胞周期明显阻止于G1期，而K562细胞凋亡率为9.97%. MTT检测表明RES对人肝癌HepG2细胞有剂量效应关系. 但RES对人K562细胞的生长无明显的抑制作用. 结论：RES通过诱导HepG2细胞凋亡抑制肿瘤的生长，并有剂量效应关系. 同时RES对肿瘤细胞的抑制及诱导凋亡的作用有细胞选择性.
【关键词】 白藜芦醇；细胞凋亡；细胞周期；HepG2；K562
0引言
白藜芦醇（Resveratrol，RES）是一种重要的活性化合物，广泛存在于植物中. 具有顺式及反式两种结构,反式具有生物学活性. 其化学名为反3，4′，5三羟基芪，它所具有的对心血管疾病和肿瘤的预防和保健作用已在国际上引起广泛的关注及深入研究. RES有广泛抗癌和防癌作用，在致癌作用的3个主要阶段均［1］具有化学防癌活性. 因此，我们以RES分别诱导人肝癌HepG2和慢粒K562肿瘤细胞凋亡的影响及作用来探讨RES诱导肿瘤细胞凋亡和细胞周期阻滞的机制.
1材料和方法
1.1材料
人肝癌HepG2和慢粒K562细胞由第四军医大学生物化学与分子生物学教研室第四实验室冻存. RES购于Sigma公司,溶于二甲基亚砜（DMSO, 购于Sigma公司）,浓度稀释为5×106 mol/L,-20℃备用. RPMI 1640和新生牛血清购自Invitrogen公司. MTT购于华美生物工程公司，溶于1×PBS（10 mmol/L, pH 7.4）使浓度为5 g/L，0.22 μm的微孔过滤除菌后4℃备用. DNA荧光染料(PI和Annexin V)购于Coulter公司，4℃备用.
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