| | | 养阴抗毒胶囊2号对阴虚大鼠糖皮质激素受体蛋白表达的影响
| | 医学检验论文 作者：丁鑫，侯颖，雎岩,史恒军
【关键词】 养阴抗毒胶囊2号；糖皮质激素类；阴虚；糖皮质激素受体；大鼠
【Abstract】 AIM: To observe the effect of Nourishing Yin Antitoxic Capsule 2 (YC2) on the expression of glucocorticoid receptor proteins (GR) in Yin deficiency rats induced by hydrocortisone, and to investigate how the YC2 relieves the side effects of Glucocorticoid. METHODS: Thirtytwo SD rats were randomly allocated to 4 groups: normal control group, Yin deficiency model control (YD) group, YC2 group and Guifu Dihuang Balls (GFB) group. Radioimmunological method was used to observe the contents of corticosteroid (CORT). Immunohistochemistry was used to observe the expression of GR in the liver cell nucleus. RESULTS: Compared with the normal group, the contents of CORT were higher in YD group (P<0.05), which was （9.92±0.69） μg/L, and the expression of GR in the liver cell nucleus was lower for the optical density ratio of immunohistochemistry stain (0.316±0.017, P<0.05）. Compared with the YD group, the contents of CORT in YC2 group were lower（P<0.05）, which was (7.28±0.72) μg/L, and the expression of GR in the liver cell nucleus was higher for the optical density ratio of immunohistochemistry stain (0.342±0.013, P<0.05）; the contents of CORT in GFB group were little lower（P>0.05）, and the expression of GR in the liver cell nucleus was little higher （P>0.05）. CONCLUSION: In this experiment, the contents of CORT in YD group were obviously higher than the normal group, and the expression of GR in the liver cell nucleus was lower. The YC2 can increase the expression of GR in the liver cell nucleus, and improve the hyperfunction of hypothalamicpituitaryadrenal (HPA) axis.
【Keywords】 Nourishing Yin Antitoxic Capsule 2; glucocorticoids; Yindeficiency; glucocorticoid receptor; rats
【摘要】 目的医学检验论文：观察养阴抗毒胶囊2号（YC2）对阴虚大鼠模型肝组织糖皮质激素受体（GR）蛋白表达的医学检验论文影响和对减轻激素副作用机理. 方法：SD大鼠32只，随机分为空白对照组、阴虚模型组（YD）、YC2组和桂附地黄丸组（GFB）. 大鼠在中药预处理及造模给药9 d后处死，用放射免疫方法检测各组血浆皮质醇（CORT）含量，免疫组织化学染色技术测定肝组织细胞核内GR表达情况. 结果：YD组CORT含量为（9.92±0.69） μg/L，与空白对照组相比，明显升高，肝组织细胞核内GR染色光密度比值为0.316±0.017，明显减少（P<0.01）. YC2组血浆CORT含量为（7.28±0.72）μg/L，均不同程度低于YD组（P<0.05），同时肝组织细胞核内GR染色光密度比值为0.342±0.013，较YD组明显增加（P<0.05）. GFB组与YD组相比，血浆CORT含量稍低（P>0.05），肝组织核GR蛋白表达稍增高（P>0.05）. 结论：本实验中阴虚大鼠模型血浆CORT含量明显升高，肝组织细胞核内GR表达明显减少，而YC2能提高阴虚大鼠肝组织细胞核内GR表达，改善下丘脑垂体肾上腺(HPA)轴功能亢进状态，减轻激素副作用.
【关键词】 养阴抗毒胶囊2号；糖皮质激素类；阴虚；糖皮质激素受体；大鼠
养阴抗毒胶囊2号(yin antitoxic capsule 2, YC2)已临床应用多年，其作用为滋阴解毒，清热活血. 它对大剂量外源性糖皮质激素(glucocorticoid, GC)所致的阴虚症状有明显的改善作用. 前期试验证明YC2能有效的保护模型动物肾上腺皮质网状带不受大剂量外源性GC的破坏，明显改善GC作用下下丘脑垂体肾上腺(hypothalamicpituitaryadrenal, HPA)轴功能亢进状态，对GC所致的各种阴虚症状有很好的疗效［1-2］. 我们在前期研究的基础上继续观察该方对应用GC所致阴虚大鼠糖皮质激素受体(glucocorticoid receptor, GR)的影响及调节作用，为临床进一步应用YC2治疗激素副反应提供了实验依据.
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