| | | 放射性125I粒子照射小鼠膀胱癌模型的实验研究
| | 评职论文下载 作者： 杜明华，高立宁，姚茂银，郭金和
【关键词】 碘
【Abstract】 AIM: To get insights into the biological effect of implanting 125I seeds to treat bladder cancer model of T139 mice and to provide an experimental basis for clinical utilization of 125I seed brachytherapy for bladder cancer. METHODS: Eight mice models bearing T139 bladder cancer were divided into 2 groups (experimental, 4; control, 4) at random. 125I seeds were implanted into the tumor in experimental group while the empty seed was implanted in the control group at the same position as the experimental group. The experimental mice were executed after 24 d. Three tissues were cut from different positions according to the different distance (0.6, 1.2, 1.8 cm) from the implanted 125I seeds and the tumor cells were primarily cultured and the pathomorphological changes, growth and proliferation of tumor cells were dynamically recorded. Tumor cell count, efficiency and inhibitory rate of colony formation were calculated. RESULTS: ① The cell number of experimental group was obviously fewer than that of control group at the same time point. There was a significant difference in the number of cultured cells from different positions of experimental group. ② The inhibitory rate of colony formation was 41.84%. ③ Variance analysis of total cloning efficiency of the cells from different positions showed a significant difference between experimental group and control group (P<0.01), and among different positions of experimental group (P<0.01). CONCLUSION: The γ radiation from implanted 125I seeds directly destroys tumor cells within the effective range. The radiation dose and killing effect of γray decreased with the increasing distance from 125I seeds.
【Keywords】 125I seed; bladder neoplasms; interstitial implant; mice
【摘要】 目的评职论文下载： 揭示125I粒子照射膀胱癌的评职论文下载生物学效应，为临床应用125I粒子治疗膀胱癌提供实验依据. 方法： 把T739近交系小鼠膀胱低分化移行细胞癌动物8只随机分为两组，实验组和对照组，每组4只. 在小鼠瘤体内植入MSI125型125I粒子，在对照组小鼠瘤体内相应部位植入粒子空壳，继续饲养24 d后处死. 在距粒子植入部位0.6, 1.2, 1.8 cm的小鼠肿瘤内分别取3块组织，分离后行癌细胞培养，显微镜下动态观察细胞病理形态变化及生长、增殖状况. 统计克隆形成率，计算克隆形成抑制率. 结果： ① 实验组细胞数量明显少于对照组. 实验组不同部位的培养细胞数量有较明显差异. ② 实验组克隆形成抑制率为41.84%. ③ 两组不同部位所取组织细胞的总体克隆形成率有显著差异（P<0.01），实验组不同部位间亦有显著差异(P<0.01). 结论： 植入的125I粒子主要是靠γ射线的穿透力在有效射程内直接杀伤癌细胞，随着距离延长，辐射剂量减少，γ射线对癌细胞杀伤作用下降.
【关键词】 碘125粒子；膀胱肿瘤；组织间植入；小鼠
膀胱癌作为一种高发的恶性泌尿系肿瘤，其放疗实践与研究只局限于外放疗和腔内放疗，对于组织间种植治疗的应用较少［1］，其基础性研究也鲜见报道. 我们通过对125I粒子植入治疗膀胱癌产生的生物学效应的实验研究，揭示125I粒子的杀瘤效应，为临床应用125I粒子治疗膀胱癌提供实验基础.
1材料和方法
1.1材料T739近交系普通清洁级小鼠4 wk龄8只，雌雄随机，由同济大学医学院实验动物中心提供. 含小鼠可移植性膀胱移行细胞癌株（BTT739）的荷瘤T739小鼠2只作瘤源，由上海市第一人民医院病理科提供. MSI125型125I粒子由中国原子能科学研究院同位素研究所提供. 摄像显微镜(Nikon Fx35DX，日本尼康公司). 二氧化碳孵育箱（NAPCO，U.S.A）.
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