| | | 不同浓度葡萄糖对MG63细胞株TRAIL表达的影响
| | 医学检验论文 作者：周玮，姬秋和，张南雁，黄威权
【关键词】 葡萄糖
Effects of different concentrations of glucose on expression of TRAIL in MG63 cells
【Abstract】 AIM: To observe the regulative effects of different concentrations of glucose on the expressions of tumor necrosis factorrelated apoptosisinducing ligand(TRAIL),osteoprotegerin(OPG),and the ligand of osteoprotegerin(OPGL) in osteosarcoma MG63 cells,and to study the role of nonphysiological concentration of glucose in pathogenesis of diabetic osteoporosis. METHODS: MG63 cells were incubated with glucose at the concentration of 5.5, 16.7, 33.3 mmol/L for 24 h respectively.The expressions of TRAIL, OPG and OPGL mRNA were examined by reverse transcriptase(RT)PCR.Expression and distribution of TRAIL in MG63 cells were investigated by immunohistochemical method. RESULTS: The mRNA expressions of TRAIL and OPGL in MG63 cells increased in the order of control group, 5.5, 16.7, 33.3 mmol/L glucose groups（P<0.05）,and the expression of OPG mRNA decreased in the same order（P<0.05）.TRAIL mRNA level in 33.3 mmol/L group was significantly higher than that in control group［(1.004±0.070) vs (0.740±0.023), P<0.05］, and the intensity of immunostaining for TRAIL was stronger than that in control group. CONCLUSION: High concentration of glucose could lead to the increasing expression of some boneresorbing cytokines such as TRAIL and OPGL in osteoblasts but the decreasing expression of OPG, which may be one of the key pathogenetic factors of diabetic osteoporosis.
【Keywords】 glucose; MG63 cell; tumor necrosis factorrelated apoptosisinducing ligand
【摘要】 目的医学检验论文： 观察不同浓度葡萄糖环境下人成骨肉瘤MG63细胞株肿瘤坏死因子相关凋亡诱导配体（TRAIL）及其护骨素（OPG）、护骨素配体（OPGL）的医学检验论文表达，探讨糖尿病骨质疏松症的发病机制. 方法： 用不同浓度葡萄糖(5.5, 16.7, 33.3 mmol/L)分别刺激培养的MG63细胞24 h， RTPCR法检测TRAIL， OPG， OPGL mRNA的表达，免疫组织化学法检测MG63细胞中TRAIL的表达及分布. 结果： 葡萄糖对MG63细胞中TRAIL， OPGL mRNA的表达，均按照对照组、5.5 mmol/L组、16.7 mmol/L组、33.3 mmol/L组顺序递增（P＜0.05），OPG mRNA的表达按照此顺序递减（P＜0.05）. 33.3 mmol/L组TRAIL mRNA的水平明显高于对照组［(1.004±0.070) vs (0.740±0.023), P＜0.05］, TRAIL免疫反应阳性物质密度也明显高于对照组. 结论： 高糖环境可能导致成骨细胞中TRAIL和OPGL表达增多，OPG的表达减少，这可能是糖尿病骨质疏松症的重要发病机制之一.
【关键词】 葡萄糖；MG63细胞；肿瘤坏死因子相关凋亡诱导配体
0引言
糖尿病易并发骨质疏松症已被公认, 糖尿病对骨代谢的影响主要表现为骨吸收增加,骨形成减少与缓慢,使骨矿物质含量减少和骨质疏松［1］. 确切的细胞机制尚未阐明. 肿瘤坏死因子相关凋亡诱导配体（tumor necrosis factorrelated apoptosisinducing ligand, TRAIL）是新发现的肿瘤坏死因子超家族的成员，可表达于人体的许多组织，对许多肿瘤来源的细胞具有很强的杀伤作用［2-3］，可介导病毒引起的凋亡［4-5］，并在一些自身免疫疾病中起作用［6］. 但在糖尿病骨质疏松发病中是否起作用，目前少见报道. 我们观察不同浓度葡萄糖环境下具有人成骨细胞表型特征的MG63细胞株中TRAIL及其护骨素（osteoprotegerin, OPG）、护骨素配体（osteoprotegerin ligand, OPGL）的表达情况, 进一步探讨糖尿病骨质疏松症的发病机制.
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