| | | 人微小病毒B19感染与Kikuchi病的相关性
| | 医学实践论文【关键词】 病毒
Relationship between human parvovirus B19 infection and KikuchiFujimoto disease
【Abstract】 AIM: To explore the relationship between parvovirus B19 infection and idiopathic KikuchiFujimoto disease (KFD). METHODS: Tissue blocks from lymph nodes of 32 idiopathic KFD patients and from normal lymph nodes of 13 normal controls were detected for parvovirus B19 DNA and viral proteins by in situ hybridization and immunohistochemical techniques in a doubleblind manner. In situ hybridization and immunohistochemical doublestaining were used to determine the type of virusinfected cells. RESULTS: In situ hybridization and immunohistochemistry revealed that the positive rates of B19 DNA and viral proteins were 71.9% and 59.4% respectively in the 32 idiopathic KFD patients. In the control group, the positive rates were 30.8% and 23.1%, respectively. Statistical analysis indicated that the positive rates of B19 DNA and proteins differed significantly between the idiopathic KFD patients and the controls (P=0.011 and P=0.027 respectively). The B19 virusinfected cells were mainly lymphocytes and a small number of histocytes. CONCLUSION: Lymph nodes from idiopathic KFD patients have higher parvovirus B19 infection rates than those from controls. Parvovirus B19 infection may play an important role in the pathogenesis of idiopathic KFD.
【Keywords】 histiocytic necrotizing lymphadenitis; parvovirus B19; in situ hybridization; immunohistochemistry
【摘要】 目的医学实践论文：研究人微小病毒B19感染与组织细胞性坏死性淋巴结炎(KFD)的医学实践论文相关性. 方法：采用双盲法在32例KFD患者及13例正常对照淋巴结活检组织中用原位杂交和免疫组织化学染色方法检测B19病毒基因组DNA和病毒蛋白. 并采用原位杂交和免疫组化双标记法确定病毒感染的细胞类型. 结果：在32例KFD患者淋巴结中，B19病毒DNA和病毒蛋白阳性率分别为71.9%和59.4%，而在对照淋巴结中，二者阳性率分别为30.8%和23.1%. 统计学分析显示B19病毒基因组DNA和病毒蛋白在KFD中有更高感染率(P值分别为0.011和0.027). 病毒感染的细胞大部分为淋巴细胞，少数组织细胞也是病毒感染的靶细胞. 结论：微小病毒B19在KFD中有更高的感染率，微小病毒B19与KFD的发病有密切关系.
【关键词】 组织细胞性坏死性淋巴结炎；微小病毒B19；原位杂交；免疫组织化学
0引言
组织细胞性坏死性淋巴结炎(KikuchiFujimoto Disease, KFD)由Kikuchi和Fujimoto于1970年首先报道. 是一种主要累及淋巴结的自限性全身性疾病，世界各地均有报道，但以东方青年女性多见. 其病理特征主要为淋巴组织散在灶片状坏死，伴有大量组织细胞浸润，易与淋巴瘤混淆，病因尚不清楚. 曾有研究者发现在系统性红斑狼疮和嗜血细胞综合症伴发KFD样淋巴结改变患者中检测到人微小病毒B19［1-2］，但对于B19病毒与原发性KFD的关系，仅Chiu等［3］用免疫组化的方法检测了10例KFD患者的淋巴结活检标本，但未得到阳性结果. 在此，我们观察了人微小病毒B19基因组DNA及病毒衣壳蛋白在原发性KFD中的表达情况，以探讨二者的关系.
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