| | | 人类白细胞抗原等位基因与HBV宫内感染关系
| | 评职论文下载【关键词】 乙型肝炎病毒；宫内感染；人类白细胞抗原DR等位基因(HLA-DR)
摘要： 目的评职论文下载 研究人类白细胞抗原(HLA)-DR3、DR7、DR13、DR53等位基因与乙型肝炎病毒(Hepatitis B virus，HBV)宫内感染的评职论文下载关系，探讨HBV宫内感染的遗传易感性，确定HBV宫内感染的易感基因和保护基因。方法 采用聚合酶链反应/序列特异性引物(PCR-SSP)技术检测HBsAg阳性孕妇及其新生儿各187例外周静脉血血块中HLA-DR3、DR7、DRl3、DR53等位基因型分布及频率。结果 (1)宫内感染组孕妇HLA-DR3基因频率为2414％(7／29)，显著高于非宫内感染组孕妇的633％(10／158)(P=0007)。其余各表型比较，差异无统计学意义(P>005)。(2)宫内感染组新生儿HLA-DR3基因频率为1724％(5／29)，显著高于非宫内感染组新生儿的506％(8／158)(P=0033)。其余各表型比较，差异无统计学意义(P>005)。(3)母婴HLA-DR3同阳性在宫内感染组与宫内未感染组间比较差异有统计学意义(P=0049)；母婴HLA-DR7、DR13、DR53同阳性在宫内感染组与宫内未感染组间比较，差异无统计学意义(P>005)。结论 HBsAg阳性孕妇及其胎儿同时或任一携带HLA-DR3，易导致HBV宫内感染；HLA-DR3是HBV宫内感染的易感基因。
关键词： 乙型肝炎病毒；宫内感染；人类白细胞抗原DR等位基因(HLA-DR)
Association between HLA-DR alleles and HBV intrauterine infection
Abstract： Objective To study the association between HLA-DR3,DR7,DR13,DR53 and HBV intrauterine infection to probe into the susceptibility to intrauterine infection of hepatitis B virus and to find its predisposing genes and protecting genes.Methods HLA-DR3,DR7,DR13,DR53 were detected by the technique of polymerase chain reaction-sequence specific primers(PCR-SSP) in maternal and neonatal blood clot.Results (1) The frequency of HLA-DR was 24.14% in women with intrauterine infection,which was higher than that (6.33%) in women without intrauterine infection(P=0.007).There was no association between HLA-DR7,DR13,DR53 positive in pregnant women and HBV intrauterine infection(P>0.05).(2) The frequeney of HLA-R of newborns was 17.24% in women with intrauterine infection,which is higher that (5.06%) in women withotll infection(P=0.033).There was no association between HLA-DR7,DR13,DR53 positive in newborns and HBV intrauterine infection(P>0.05).(3)There was significant association between HLA-DR3 concordance and HBVintrauterine infection(P=0.049).There was no association between DR7,DR13,DR53 concordance and HBV intrauterine infection(P>0.05).Conclusion HLA-DR3 carriers of pregnant women and their newborns have susceptibility to HBV intrauterine infection.HLA-DR3 is the predisposing gene of HBV intrauterine infection.
Key words： HBV；intrauterine infection；HLA-DR antigen
肝炎是一种严重危害人类健康的世界性传染病，我国是乙肝高发区，乙肝病毒的母婴传播不仅造成人群中众多的HBsAg携带者，而且是引起成人慢性肝炎、肝硬化以及肝癌的重要因素。迄今，多数学者认为，人类白细胞抗原DR等位基因(HLA-DR)与乙型肝炎病毒(HBV)感染密切相关，而HLA-DR等位基因与HBV宫内感染的关系争议较多。本文通过研究HLA-DR3、DR7、DR13、DR53与HBV宫内感染的关系，探讨HBV宫内感染的遗传易感性，为防制HBV宫内感染提供理论依据。
1 对象与方法
11 对象 以2003年6月～2004年11月太原市省各市级医院筛检，并在太原市传染病院妇产科进行产前检查及分娩的HBsAg阳性孕妇及其新生儿各187例作为研究对象。并根据新生儿有无宫内感染，分为宫内感染组(29例)和宫内未感染组(158例)，2组在年龄分布、文化程度、职业构成等均衡可比。
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