| | | 纸片法与培养发酵法检测大肠菌群结果对比分析
| | 教育论文下载关键词：纸片法;大肠菌群;灵敏度;特异度
摘要:目的教育论文下载 探讨改进纸片法检测大肠菌群的教育论文下载判断标准。 方法 对纸片法检测大肠菌群的纸片反应结果状况进行详细记录后，将纸片以无菌操作转入单料乳糖胆盐发酵管中，用发酵法来证实其大肠菌群的阴阳性结果，再作统计分析。 结果 按GB14934-94判断纸片法大肠菌群的阴阳性，其灵敏度为73.3%，特异度为99.2%;若按作者提出的标准进行判断，其灵敏度为97.9%，特异度为90.3%。二者比较灵敏度有显著性差异（P<0.05）。消毒剂的残留可能是导致纸片法结果判断出偏差的主要原因。 结论 据本实验结果分析，建议改进和完善纸片法大肠菌群的判断标准，以更准确地反映大肠菌群检出的真实情况。
关键词:纸片法;大肠菌群;灵敏度;特异度
Comparison of results of slip method with culture ferment method in detection of E.coli.
Abstract:Objective To compare the slip method with culture ferment method in the detection of E.coli and for improving the accuracy of judgment of detection results. Methods Slip method was used for monitoring the contamination status of cooking uten-sils and the slips were put into lactose bile salts broth tubes for confirmation of the detection results by using ferment method.The re-sults were statistically analyzed. Results Based on the judgment standard of GB14934-94，the sensitivity and specificity of the slip method were73.3%and99.2%respectively.But the sensitivity and specificity were，based on the standard proposed by the author，97.9%and90.3%，respectively.The difference of the sensitivities was significant（P<0.05），and the main cause of dif-ference in judgment of the results was the residual of the disinfectant. Conclusion Thus it is suggested that the standard for judg-ment of results in detection of E.coli be modified so as to improve the accuracy of detection.
Key words:Slip method;E.coli;Specificity;Sensitivity
纸片法检测大肠菌群（简称纸片法，下同），1994年被正式列入餐（饮）具消毒效果监测的国家标准 ［1］ （下称国标或GB）。随后，纸片法被各级疾病预防控制中心卫生检验广泛使用，它以经济、方便、快捷等优点给现场监测带来了极大的便利，不但节省了大量人力、物力，而且还扩大监测覆盖面提供了技术保障。但在这几年的实践经验中，发现在纸片法的结果观察中出现许多与国标中明示的结果判断不一致的情况。为此，于2004年1～6月间对江门市区餐具、茶具监测的581份标本采用纸片法和培养发酵法（下称发酵法）进行大肠菌群检测平行对比实验研究，现将结果分析报告如下。
1 材料与方法
1.1 材料 大肠菌群纸片由深圳市博卡生物技术有限公司提供;乳糖胆盐发酵液、乳糖复发酵液、伊红美蓝培养基（EMB）和革兰氏染液均由杭州天和微生物试剂有限公司生产。以上培养基和试剂均在有效期内使用。试验样品包括碗、盘、筷、汤匙等餐具，均来源于我市各大中小型餐厅。
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