| | | 钩藤散浸膏对亚急性衰老模型小鼠的影响
| | 西医学 毕业论文黄厚才1，胡元亮2，胡春萍1，彭蕴茹1，丁永芳 【摘要】 目的西医学 毕业论文 观察钩藤散浸膏对衰老模型小鼠智力、乙酰胆碱酯酶（ACHE）、抗氧化酶（SOD）、自由基（MDA）的西医学 毕业论文影响，探讨其抗衰老作用及机制。方法 小鼠随机均分为6组，除正常对照组外，各组每天皮下注射D-半乳糖（0.05g/kg），连续注射6周。从注射的第7天开始，3个实验组同时灌胃高、中、低剂量的钩藤散浸膏，阳性对照组灌以维生素E（0.05g/kg）模型对照组与正常对照组每天灌胃等量的蒸馏水，连续给药5周后测定小鼠的训练成绩、记忆成绩及血清SOD、ACHE和脑组织MDA的含量变化。结果 实验组的训练成绩显著（P＜0.05），测试成绩极显著（P＜0.01）好于、血清SOD、ACHE活力极显著（P＜0.01）高于、脑MDA含量极显著（P＜0.01）低于模型对照组；与正常组比，除ACHE偏高外，其余各指标相近。结论 钩藤散能提高抗氧化能力，降低自由基含量，增强胆碱能神经系统功能，从而防止衰老。
【关键词】 D-半乳糖；钩藤散；衰老；小鼠
【Abstract】 Objective To observe the effects of Goutengsan extract on memory，malondiadehyde（MDA），superoxide dismutase（SOD），ACHE in mice to study therapeutic effects and mechanism of Goutengsan on aging model.Methods Mice were randomly divided into 6 groups. All groups，except the control，were administrated （ip） with D-galactose （0.05g/kg） for successive 6 weeks. The 3 experimental groups were administrated （ig） with high，middle and low dosages of Goutengsan extract respectively after 7th day of the injection of D-galactose whereas vitamin-E at 0^05g/kg body weight served as a positive control. The model and control groups were daily adminisered with D.D.W. for continuous 5 weeks. After treatments，the SOD，ACHE activity in serum and MDA in brain of rats were determined，the training score and memory grade were also recorded.Results Goutengsan extract effectively increased the training scores and memory grades. Also，the serum SOD，ACHE activities in Goutengsan extract-treated mice were higher than that in control group. However，the brain MDA content in Goutengsan extract-treated mice was lower than that in control group.Conclusion This data suggested that Goutengsan extract had a significant anti-oxidative effect by reducing the free radicals as well as an anti-aging effect through the enhancement of cholinergic nerve system.
【Key words】 D-galactose; goutengsan; aging; mice
老年痴呆是威胁人类健康的重大疾病之一，对其的防治一直是全球关注的焦点。钩藤散源于《本事方》，由钩藤、石膏、陈皮、麦冬、半夏、茯苓、人参、防风、生姜、甘草、菊花等11味药组成，原为治疗肝厥头晕之证的经典方剂。本次实验进行了钩藤散浸膏的抗衰老实验，以观察钩藤散浸膏在抗衰老方面的影响及机制。
1 材料
1.1 药品与试剂 （1）钩藤散：由本院中药制剂室按传统方法水提、浓缩、消毒等制成浸膏。临用前每19ml钩藤散浸膏加蒸馏水配至100ml，变成0.5g生药/ml清膏溶液，实验中所用剂量均以生药量计。（2）维生素E胶丸：厦门鱼肝油厂（批号：10842002）。用前将维生素E胶丸用蒸馏水按10粒/100ml蒸馏水配成溶液。（3）D-半乳糖：国药集团化
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