| | | 排石合剂质量标准研究
| | 西医学 免费毕业论文摘 要: 排石合剂为我院新开发的西医学 免费毕业论文一种制剂，以金钱草为君药辅以怀牛 膝和车前子等组成。可用于治疗尿路感染、血尿和泌尿系统结石等疾病。建立本品的西医学 免费毕业论文质量标 准为质控提供依据。处方中的黄酮和皂苷用定性方法鉴别，金钱草和怀牛膝用TLC进行鉴别 ，其中的总黄酮按《中华人民共和国药典》1995年版所载比色法测定实验表明在0.008 ～0.048 mg/mL范围内浓度与吸收度呈良好的线形关系，其低、中、高浓度的加样回收率分 别为100.39%、102.36%和101.17%，本方法简便、快速且重现性好。
Studies on the Quality Standard of Pai shi Mixture
Abstract Pai shi mixture is a newly developed herbal prepara tion composed of Glechoma longituba (Nakai) Kupr. as the main compo nent supplement ed by Achyranthes bidentata, Plantago asiatica and several other minor ingredients. It was proposed for the treatment of urinary infection, hem at uria and urinary calculi. The object of the present study is to establish a sta n dard for the quality control of the product. The presence of flavonoids and sap o nins were varified by specific qualitative identification. G. longitub a and A. bidentata were distinguishing by TLC. Content o f total flavonoids was determined b y colorimetry as described in the 1995 edition of Chinese Pharm acopoeia. The plot of concentration against absorption gave a straight line ([ W TBX〗r=0.999 6) over the range of 0.008～0.048 mg/mL. The averag e recovery of low, middle and high concentrations were 100.39%, 102.36% and 10 1.17% respect ively. The method proved to be simple, rapid and reproducible.
Key words Pai shi Mixture total flavonoid colorimetry Glechoma longituba (Nakai) Kupr.
排石合剂是我院的一种自制制剂，由金钱草、怀牛膝、车前子等中药组成，具有利尿排石、 清热消炎、凉血止血等功效，用于治疗泌尿系统结石、尿路感染、血尿等疾病，疗效很好。 为了制定控制内在质量的标准，我们根据处方中药物的性质，对其中所含的黄酮类、皂苷类 成分及 金钱草、怀牛膝等药物分别做了鉴别试验，并进行了总黄酮的含量测定，从而为质量标准的 制定提供了依据。
1 仪器与试药
UV-210PC 型紫外分光光度计(日本岛津)；紫外光灯(上海顾村电器厂)；硅胶G板(青岛海洋 化工厂)；聚酰胺薄膜(台州市路桥四青生化材料厂)；芦丁、齐墩果酸对照品(中国药品生物 制品检定所)；排石合剂及药材原料由我院制剂室提供。
2 鉴别试验
2.1 皂苷类成分
2.1.1 取本品适量，置于具塞试管中，密塞，强力振摇，产生大量泡沫并且在20 min内 不明显减少。
2.1.2 取本品10 mL于具塞试管中，加无水乙醚10 mL提取2 次，合并乙醚层，减压蒸干 ，加少许冰醋酸溶解，转移至试管中，沿管壁缓缓加入硫酸适量，可见冰醋酸层为红色，中 间有红褐色环，硫酸层有黄绿色荧光。
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