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从噬菌体表面展示环状7肽肽库中筛选葡萄球菌B型肠毒素抑制剂
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【医学毕业论文下载】 作者:李韩平,周宏,郑玉玲,邓小红,马茹,姜永强【关键词】 葡萄球菌B型肠毒素Screening inhibitor of Staphylococcal enterotoxin B from random circular 7mer peptide library displayed on phage 【Abstract】 AIM: To obtain the specific peptide which can bind with SEB and inhibit SEBs enterotoxin activity by panning from the random circular 7mer polypeptide libraries. METHODS: The affinity of peptide and the competitive activity of the synthetic peptide with that of antiSEB monoclonal antibody (McAb) were detected respectively by PhageELISA and competition ELISA. Animal experiment was performed to assess the suppressive effect of the screened peptide on the toxicity of SEB and its enterotoxin. RESULTS: The circular 7mer peptide obtained by panning suppressed the multiplication of spleen lymphocytes in mice. At the ratio of 160:1 (SEB: synthetic 7mers peptide), the peptide protected the litt
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