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不同浓度BME对大鼠胚胎脊髓神经干细胞增殖和分化的影响
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【防疫医学论文】【关键词】 神经干细胞Effect of βmercaptoethanol on proliferation and differentiation of neural stem cells from embryonic rats
【Abstraet】 AIM: To explore the effects of different concentrations of βMercaptoethanol (BME) in DFI2 containing 10 mL/L fetal cow serum (FCS) on the proliferation and differentiation of neural stem cells from rat embryonic spinal cord. METHODS: We isolated neural stem cells (NSCs) from the spinal cord and purified them through 23 generations. We then used different concentrations of BME containing 10 mL/L FCS to induce NSCs in vitro, observed the cells in three phases, identified the differentiation using immunocytochemistry by GFAP and NSE 24 h later, and analyzed the results. RESULTS: Proliferation was observed in NSCs induced either by BME (0.5 mmol/L or l mmol/L) and FCS or only by FCS. The differentiation rate of NSCs to neuron
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