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巨细胞病毒抗原分支肽的合成与鉴定
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【医学论文代发】 作者：杨赛，刘陶，杨浩，韩锋产， 陈五岭【关键词】 人巨细胞病毒Synthesis and identification of CMV branched peptide
【Abstract】 AIM: To investigate the synthesis of human cytomegalovirus antigenic branched peptide. METHODS: The linear peptide and branched multiple peptide with human cytomegalovirus antigenic epitope were synthesized with solid phase chemistry respectively and purified by HPLC. The two peptides molecular masses were obtained by mass spectrum and then identified with quality control serums. RESULTS: The molecular mass of the linear peptide was 793.8 and that of branched multiple antigenic peptide was 7127.77, both consistent with the theoretic molecular mass. The branched peptide differentiated quality control serums properly through ELISA. CONCLUSION: The synthesis of cytomegalovirus antigenic branched peptide greatly facilitates the previous way for the prepar
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