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抑制性消减杂交技术筛选乙型肝炎病毒核心蛋白相互作用蛋白编码基因C1的反式调节基因
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【药学毕业论文】 作者：蔺淑梅,张树林,成军,刘敏,郭江,张黎颖,杨媛【关键词】 抑制性消减杂交；反式激活；克隆;,乙型肝炎病毒【Abstract】 AIM： To clone and identify human genes transactivated by human gene C1 encoding protein C1 interacting with hepatitis B virus (HBV) core antigen by constructing a cDNA subtractive library with suppression subtractive hybridization (SSH) technique. METHODS： SSH and bioinformatics techniques were used for screening and cloning of the target genes transactivated by C1 protein. The mRNA was isolated from HepG2 cells transfected with pcDNA3.1(-）C1 and pcDNA3.1(-） empty vector, respectively, SSH method was employed to analyze the differentially expressed cDNA sequence between the 2 groups. After restriction enzyme Rsa I digestion, small sizes cDNAs were obtained. Then tester cDNA was divided into 2 groups and ligated to the specific adaptor 1 and adaptor 2, respectively. After tester cDNA was hybridiz
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