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应用细胞内PCR技术构建及筛选白癜风噬菌体抗体库
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【西药学科研论文】 作者：刘玲，田艳丽，李春英，高天文【关键词】 白癜风；聚合酶链反应；噬菌体抗体库；自身抗体Construction and screening of human vitiligo phage autoantibody library based on incell PCR
【Abstract】 AIM: To obtain in situ pairing of the variable region genes of the heavy and light chains of vitiligo patients lymphocytes, then construct and screen a human ScFv phage antibody library. METHODS: We amplified in situ pairing of the variable region genes of the immunoglobulin heavy and light chains using the incell PCR together with Crerecombination, constructed and panned a human ScFv phage antibody library, and screened it with pigment cell suspension, then detected the activity of positive clones. RESULTS: We constructed an antibody library of 3×l06 in size, and obtained one specific autoantibody against melanocyte membrane antigens. CONCLUSION: Incell PCR together with human phage antibo
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