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人羧肽酶A1活性中心的毕赤酵母可溶表达
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【西药不良反应论文】 作者：易静，郝晓柯，苏明权，岳乔红，刘家云，张瑞，雷迎峰【关键词】 人羧肽酶【Abstract】 AIM: To express the human carboxypeptidase A1 (CPA1) active center protein in soluble form in pichia yeast. METHODS: Human CPA1 active center gene was amplified from pGEXCPA1 recombinant plasmid and subcloned into pPIC9K yeast expression vector. Recombinant vector pPIC9KCPA1 active center was transformed into pichia SMD1168 by electroporation. Positive recombinant yeast strains were screened through nutrition defect and G418 resistance. The integrated multicopy recombinant yeast strains were induced for expression. RESULTS: The yeast recombinant expression vector pPIC9KCPA1 active center was constructed and recombinant yeast strains which integrated over 16 recombinant vector copies were obtained. The human CPA1 active center protein was expressed in soluble form in pichia yeast after screened with G418. CO
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