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硫氧还蛋白融合表达系统表达的小鼠内皮抑素之纯化和生物学活性鉴定
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【西药学类毕业论文】 作者:马卫列,刘芳,何承伟,何振辉【关键词】 蛋白Purification of mouse endostatin in thioredoxin fusion expression system and identification of its biological activity 【Abstract】 AIM:To purify mouse endostatin by using thioredoxin fusion expression system and to identify its biological activity. METHODS: The pThioHisendo recombinant plasmid was transformed into BL21. After induction with IPTG, thioredoxinendo fusion protein was expressed in BL21 and the product was identified as inclusion body by SDSPAGE. The expression product was purified by affinity chromatography through Nicolumn. The purified protein was detected by chicken chorioallantoic membrane(CAM) angiogenesis inhibitory assay and endothelial cell proliferation inhibitory assay. RESULTS: High purity thioredoxinendostatin fusion protein was obtained by SDSPAGE analysis. The purity of the protein was over 95%. The
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