| | | 褪黑素对急性睡眠剥夺大鼠行为的影响及机制
| | 西医学 教育论文下载 作者：刘芳娥，刘利兵，陈健康，陈志扬，杨芳，化前珍，曲萍
【关键词】 褪黑素
Effect of melatonin on behavior of rats of acute sleep deprivation and its working mechanism
LIU FangE1,LIU LiBing1,CHEN JianKang1,CHEN ZhiYang1,YANG Fang1,HUA QianZhen2,QU Ping1
1Center of Basic Medicine Teaching Experiment, School of Basic Medicine, 2School of Nursing, Fourth Military Medicine University, Xian 710033, China
【Abstract】 AIM: To explore the effect of melatonin (MT) on the behavior of rats with acute sleep deprivation (SD) and the related biomechanism. METHODS: Twentyfour Sprague Dawley rats were divided into 3 groups: control group (SD + sodium chloride), experimental group 1 (SD + MT, 5 mg/ kg) and experimental group 2 (SD + MT, 15 mg/ kg). The rats were injected intraperitoneally with MT or sodium chloride one time a day for 1 week. SD was induced in the rats housed on small platform over water. After 72 h SD, the rats behavior was measured by open field test and then malondialdehyde (MDA) and nitric oxide (NO) contents in cerebral cortex and hippocampus of the rats were measured by TBA and Griess method respectively. RESULTS: The excitability and explorative behavior of the 2 experimental groups in the open field significantly decreased compared with those of the control group (P<0.05, P<0.01). There was a doseeffect relationship in the experimental groups. The contents of NO or MDA in cerebral cortex and hippocampus of the experimental groups were lower than those of the control group（P<0.01）. CONCLUSION: Melatonin depresses the excitability in the rats of acute sleep deprivation, possibly by inhibiting the increase of NO and MDA in cerebral cortex and hippocampus of the rats.
【Keywords】 melatonin; sleep deprivation; behavior; NO; MDA
【摘要】 目的西医学 教育论文下载：探讨褪黑素对急性睡眠剥夺（SD）大鼠行为的西医学 教育论文下载影响及可能的作用机制. 方法：将Sprague Dawley 24只大鼠随机分为3组，对照组给予SD和生理盐水腹腔注射，2个实验组分别给予SD和褪黑素5 mg/kg和15 mg/kg腹腔注射，连续给药7 d后，采用小平台水环境法建立大鼠SD模型，SD 72 h用旷场反应箱测试大鼠的行为，用试剂盒检测大鼠大脑皮层和海马中一氧化氮（NO）和丙二醛（MDA）含量. 结果：实验组旷场反应测试大鼠兴奋性和探索性行为均明显小于对照组（P<0.05, P<0.01），有量效关系. 实验组大鼠大脑皮层和海马组织中NO和MDA含量明显低于对照组（P<0.01）. 结论：褪黑素对睡眠剥夺大鼠行为障碍有改善作用，这种作用可能与抑制睡眠剥夺大鼠大脑皮层和海马中NO及MDA的升高有关.
【关键词】 褪黑素；睡眠剥夺；行为；一氧化氮；丙二醛
0引言
睡眠剥夺（sleep deprivation，SD）不但可引起动物或人产生疲劳、情绪不良、思维紊乱、甚至抵抗力低下、而且能引起学习记忆受损和行为的改变. 随着社会的发展，人们的生活节奏越来越快，睡眠剥夺现象日益常见. 因此，探讨减少睡眠剥夺对机体不良影响的措施具有十分重要的意义. 本实验拟研究褪黑素对睡眠剥夺大鼠行为的影响及可能的作用机制，为睡眠剥夺引起的行为障碍的防治提供理论依据.
1材料和方法
1.1材料
1.1.1实验动物上海产Sprague Dawley大鼠24只，成年、雄性、健康，体质量(180±15) g(第四军医大学实验动物中心提供). 将24只大鼠随机分为3组，每组8只. 对照组：睡眠剥夺+生理盐水；实验1组：睡眠剥夺+小剂量褪黑素；实验2组：睡眠剥夺+大剂量褪黑素.
|
| | |
| | | | <<<<<全文未完>>>>> 全文字数约3157字 | |
| | |
| |
|
教育论文中心