| | | 肌苷对帕金森病小鼠模型的神经保护作用
| | 动物医学论文【关键词】 小鼠
Inosine is neuroprective against MPTPinduced Parkinsons disease in C57BL mice
【Abstract】 AIM: To study the neuroprotective effects of inosine on 1methyl4phenyl1,2,3,6tetrahydropyridine（MPTP）induced Parkinsons disease（PD） in C57BL mice. METHODS: The PD models were formed with intraperitoneal injections of MPTP. Inosine was administered intraperitoneally to mice 30 min prior to MPTP. The effects of inosine and MPTP on the behavioral exhibition, the number of dopamine (DA) neurons in the substantia nigra and the density of tyrosine hydroxylaseimmunoreactive (THir) nerve fibers in the striatum, and the level of DA in the striatum were studied by behavioral test, immunohistochemical analysis and fluorospectrophotometry. RESULTS: The scores of locomotion, Rotarod and swim test decreased by about 45％ and 43％ and 22％, respectively. The number of DA neurons in the substantia nigra declined by about 58％, the density of THir nerve fibers also decreased, and the level of DA in the striatum declined by about 88％. Inosine, given prior to MPTP, attenuated the effects of MPTP. The scores of locomotion, Rotarod and swim test declined only by about 5％ and 11％ and 12％, respectively, the number of DA neurons in the substantia nigra declined by only about 43％ and the level of DA in the striatum declined only by about 71％. CONCLUSION: Inosine is neuroprective against MPTPinduced lesions in C57BL mice.
【Keywords】 inosine；1Methyl4pheny1,2,3,6tetrahydropyridine；Parkinson disease；nearaprotective agents；mice
【摘要】目的动物医学论文： 观察肌苷对MPTP致帕金森病（PD）小鼠模型的动物医学论文神经保护作用.方法： 用MPTP建立C57BL小鼠PD模型，在MPTP前给予肌苷，通过行为学检测（自主活动计数、Rotarod检测、游泳实验）、免疫组织化学和荧光分光光度法，观察肌苷对PD小鼠模型的行为学表现、黑质多巴胺（DA）神经元和纹状体酪氨酸羟化酶免疫反应阳性（THir）神经纤维以及纹状体DA水平的影响.结果： 给予MPTP后，小鼠行为学计数降低，自主活动计数、Rotarod检测、游泳实验分别降低约45％、43％和22％，黑质DA神经元数目减少约58％，纹状体THir神经纤维密度减低，纹状体DA水平明显降低约88％，提前给予肌苷后降低程度减轻，自主活动计数、Rotarod检测、游泳实验降低程度分别约为5％、11％和12％，黑质DA神经元数目减少约43％，纹状体DA水平降低约71％，两组相比有显著性差异（P＜001）.结论： 肌苷对MPTP所致的C57BL小鼠的神经损伤具有保护作用.
【关键词】 肌苷；1甲基4苯基1，2，3，6四氢吡啶；帕金森病；神经保护药；小鼠
0引言
帕金森病（Parkinsons disease,PD）是一种临床常见的神经系统退变性疾病，是中老年人常见的致残疾患之一.目前所知，其主要是由于黑质多巴胺（Dopamine,DA）能神经元坏死、凋亡，细胞数目减少，使黑质－纹状体通路DA释放减少，出现多巴胺乙酰胆碱失衡，患者出现静止性震颤、肌张力增高、运动减少等一系列症状，严重影响患者的生活质量.研究显示PD的发病与遗传和环境等多种因素相关，1甲基4苯基1、2、3、6四氢吡啶（1methyl4phenyl1,2,3,6tetrahydropyridine,MPTP）可以导致人类及灵长类、小鼠等多种动物产生PD样症状，为PD研究提供了新的途径.肌苷（inosine,INO）是腺嘌呤核苷和核苷酸代谢的中间产物之一，临床上长期作为能量营养剂用于多种疾病如肝脏和心血管疾病的辅助治疗.近期研究发现，肌苷在神经系统的损伤与修复中也具有重要的营养和保护作用.本研究利用常用的MPTP致C57BL小鼠PD模型，探讨肌苷在PD中的神经保护作用.
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