| | | 深低温冷冻对rhBMP
| | 药学科研论文 作者：闫小龙 李小飞 刘勇 卢强 王律
【关键词】 气管
Effect of cryopreservation on rhBMP2 induced regeneration of dogs tracheal cartilage
【Abstract】 AIM: To confirm the validity and potential application of implanting the recombinant human bone morphogenetic protein2 (rhBMP2) with collagen into the cryopreserved tracheal allografts to induce the regeneration of dogs tracheal cartilage. METHODS: Thirtytwo mongrel dogs were randomly and equally divided into 4 groups. Fivering cervical tracheal segment was harvested as the alloraft and all the grafts were wrapped with pedicled omentum. Group A was subdivided into group A1, in which RhBMP2 with collagen as a carrier was injected into the soft tissues between cartilaginous rings of the grafts, and group A2 ――the blank group. Group B, in which the grafts were cryopreserved for two months before the operation, was also subdivided into group B1 and group B2. The animals were sacrificed 8 weeks postoperation. The postmortem specimens were examined grossly and histologically. Area of new cartilage was studied in each allografts. RESULTS: Structural integrity of the allografts was better in group B than in group A. In all the groups, new cartilage was induced in the rhBMP2 injected area and more new cartilage was observed in group B1 than in group A1. CONCLUSION: rhBMP2 has the ability to induce new cartilage formation in the cryopreserved racheal allografts. Cryopreserved tracheal allografts have better new cartilage inducing effect than noncryopreserved tracheal allografts.
【Keywords】 trachea ;transplantation;cryopreservation; graft rejection ; bone morphogenetic proteins
【摘要】 目的药学科研论文： 研究应用重组人骨形态发生蛋白2 (rhBMP2)/胶原缓释系统诱导犬冷冻气管移植软骨再生的药学科研论文作用.方法： 将32只杂种犬随机等分为4组,行5个颈部气管环原位异体移植，带血管蒂大网膜包绕.A组为未冷冻组，气管环间植入rhBMP2/胶原组标记为A1组，空白对照标记为A2组；B组术前将移植体气管经深低温液氮保存2 mo，处理同A组，分别标记为B1、B2两组.所有实验犬于术后8 wk处死,行大体及病理学检查,评测各组新生软骨面积. 结果： B组犬移植气管结构保持较好、狭窄程度低.4组均有软骨再生, B1组新生软骨面积18359(5272)高于A1组14085(4926)及B2组3368(1213)，各组新生软骨面积差异有显著性(χ2 = 24010 ,df=3 ,P <001).结论： 应用rhBMP2可刺激冷冻气管移植体软骨再生，其刺激深低温冷冻犬气管移植体成骨效果优于未冷冻气管移植体.
【关键词】 气管;移植;低温保藏;移植物排斥;骨形态发生蛋白质类
0引言
气管置入保护液进行长期深低温冷冻保存，可以明显降低气管的抗原性［1,2］，避免了异体移植后的排斥反应，术后可不用免疫抑制剂，但在液氮中冷冻超过2 mo后气管软骨细胞数会降为原来的65%~75%之间［3,4］，且移植后由于缺血及轻微的慢性排斥反应，气管移植体的软骨会被进一步损伤［5］.我们根据以往气管移植系统研究的思路［6,7］,应用重组人骨形态发生蛋白－2 (recombinant human bone morphogenetic protein-2, rhBMP2)/胶原缓释系统植入深低温冷冻的犬气管移植体诱导软骨再生.试图抵抗冷冻对气管移植体的损伤, 从而最大程度保持冷冻移植体的生理结构和功能.
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