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人截短型凋亡诱导因子对HeLa细胞的促凋亡作用
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作者：张巍，张勇，孟艳玲，温伟红，薛茜，任君琳，杨安钢
【关键词】 凋亡诱导因子；,HeLa细胞；,细胞凋亡
Proapoptotic activity of truncated human apoptosisinducing factor on HeLa cells
【Abstract】 AIM: To observe the expression of truncated human apoptosisinducing factor (AIF) gene and its effect on HeLa cells. METHODS: After AIF△1-120 gene was successfully cloned, the shorter truncated human AIF gene (AIF△1-400) was constructed by deleting the Nterminal mitochondrial localization sequence(MLS), the coding sequence of flavine adenine dinucleotide (FAD) binding domain and nicotinamide adenine dinucleotide (NADH) binding domain of AIF protein．The truncated human AIF gene (AIF△1-400) was inserted into the pIRES2EGFP and pcDNA3 eukaryotic expression vectors, and the coexpression vectors were then transfected into HeLa cells with LipofectAMINE． The expression of the truncated AIF gene and its effect on HeLa cells were detected by fluorescence microscope analysis and MTT test． RESULTS: The eukaryotic expression vector containing the truncated human AIF (AIF△1-400) gene was successfully constructed． The AIF protein could be detected in the transfected HeLa cells. After transfection, typical cell apoptotic changes were observed under an electron microscope． MTT test showed that the proliferation of HeLa cells was significantly inhibited． CONCLUSION: The expression of the truncated human AIF(AIF△1-400)gene can induce the apoptosis of the transfected human HeLa cells.
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