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去辣椒素敏感性窦房结传入神经的大鼠模型的建立
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作者：张艳辉，马爱群，席雨涛
【关键词】 窦房结；,传入神经；,辣椒素；,模型
Modeling of capsaicinsensitive sinoatrial node deafferented rat
【Abstract】 AIM: To investigate the way to establish capsaicinsensitive sinoatrial node deafferented rat models. METHODS: High concentration of capsaicin was applied around rat superior vena cava. In the postoperative 3 d, 2 weeks, 1, 2 and 3 months, we observed the expressions of sinoatrial node afferent nerve transmitters ［substance P (SP) and calcitonin generelated peptide (CGRP)］ in the sinoatrial node area through immunofluorescent staining and laser confocal microscopy. RESULTS: At planned time points after the deafferentation operation and the sham operation, immunohistochemistry showed CGRP positive nerve and SP positive nerve around the sinoatrial node artery in the shamoperated rats, while there were no CGRP positive nerve and SP positive nerve in the sinoatrial node area of the deafferented rats. CONCLUSION: Application of high concentration of capsaicin around rat superior vena cava can induce sinoatrial node deafferentation, which is a repeatable and stable method.
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