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外源核苷酸对受损小鼠胸腺细胞DNA修复的影响
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【关键词】 核苷酸；DNA损伤;,DNA修复；,彗星试验
Effect of exogenous nucleotides on repair of damaged DNA in mouse thymocytes
【Abstract】 AIM: To investigate the effect of exogenous nucleotides on the repair of damaged DNA in mouse thymocytes in vitro. METHODS: Thymocytes from Kunming male mice were treated with NmethylN′nitroNnitrosoguanidine (MNNG, 10 μmol/L) to induce DNA damage. DNAdamaged cells were cultured in basic or complete RPMI 1640 medium supplemented with different levels of nucleotides (0, 0.1, 1, 10 mmol/L). DNA damage and repair were analyzed at 2, 5 h with singlecell gel electrophoresis. RESULTS: Nucleotides had no significant effect on the percentage of comet cells at 2 h in basic medium (P>0.05), but the percentage of comet cells decreased significantly in complete medium with nucleotides supplementation (P<0.01). The nucleotides supplementation significantly decreased the percentage of comet cells at 5 h (P<0.01). Comet tail length had no significant difference among groups at 2 h in basic or complete medium (P>0.05). Nucleotides of 1, 10 mmol/L decreased comet tail length at 5 h in basic medium (P<0.01) . The supplementation of 1, 10 mmol/L nucleotides decreased comet tail length at 5 h in complete medium (P<0.01). The percentage of DNArepaired cells was increased significantly with nucleotides supplementation between 2 and 5 h in basic (P<0.05) or complete medium (P<0.01). CONCLUSION: The supplementation of nucleotides can accelerate the repair of damaged DNA in mouse thymocytes in vitro. The effect of nucleotides is related with the supplemental levels of nucleotides.
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