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人白介素24基因诱导人宫颈癌Ca Ski细胞凋亡的实验研究
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【关键词】 人白细胞介素24；Ca,Ski细胞；乳头状瘤病毒,人；,E6基因；蛋白质P53；细胞凋亡
Apoptosis induction of human cervical cancer Ca Ski cells by hIL24 gene
【Abstract】 AIM: To investigate the hIL24 gene inducing apoptosis of human cervical cancer Ca Ski cells and its possible mechanism. METHODS: Ca Ski cells were transfected with hIL24 eukaryotic expression vector pcDNA3.1(+)hIL24 by lipofectamine2000. The effect of hIL24 on the transcription of HPV E6 gene was analyzed by RTPCR. The effect of hIL24 on P53 protein was analyzed by Western Blot. Apoptosis of Ca Ski cells induced by hIL24 gene was analyzed by FACS. RESULTS: Transcription level of HPV E6 gene decreased significantly, the amount of P53 protein increased significantly and the apoptosis rate of Ca Ski cells increased significantly after hIL24 transfection. CONCLUSION: HIL24 gene can inhibit the transcription of HPV E6 gene, recover the activity of P53 protein, then induce the apoptosis of Ca Ski cells.
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