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NK细胞对小鼠H
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作者：张梅,吴迪，徐汇,贺鹏程，李静，习杰英，蔡瑞波，刘亚琳
【关键词】 杀伤细胞,天然;,骨髓移植;,移植物抗宿主病;,造血重建
Effect of NK cell on GVHD and hematopoietic reconstitution after H2 haploidentical bone marrow transplantation in mice
【Abstract】 AIM: To explore the effect of natural killer (NK) cell on graftversushost disease (GVHD) and hematopoietic reconstitution after H2 haploidentical bone marrow transplantation in mice. METHODS: Murine model of H2 haplotype mismatched bone marrow transplantation was established by using Balb/c (H2d) mouse as recipient, and Balb/c (H2d)×C57BL/6(H2b)(H2d/b) mouse as donor. Lethally irradiated Balb/c (H2d) mice were transplanted with Balb/c (H2d)×C57BL/6(H2b)(H2d/b) bone marrow plus with donor peripheral T cells and/or NK cells. GVHD and hematopoietic reconstitution were observed and evaluated by clinical manifestations, pathological changes and WBC counting. RESULTS: In the bone marrow plus with T cells group, GVHD was induced in 90% mice and the recovery of the WBC needed 4 weeks averagely; but in the group plus with low concentration of NK cells, GVHD was induced in 20% mice and the recovery of the WBC was 3 weeks averagely; and in the group transplanted with high concentration of NK cells, GVHD was induced only in 10% mice and the recovery of the WBC was 3 weeks averagely. Compared with the group transplanted only with T cells, the incidences of GVHD in the later two groups decreased obviously (P<0.01), and the recovery of the WBC was in a shorter time (P<0.01). CONCLUSION: In mouse H2 haploidentical bone marrow transplantation, alloreactive NK cells can reduce the incidence of GVHD and promote hematopoietic reconstitution.
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