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siRNA干扰ADAR1表达对小鼠混合淋巴细胞培养的影响
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作者：蔡磊，霍斌亮，张巍，张福琴，赵青川
【关键词】 淋巴细胞培养试验,混合；RNA干扰；ADAR1基因；siRNA；排斥反应
Effect of siRNAmediated silencing of ADAR1 gene on mixed mouse lymphocyte culture
【Abstract】 AIM: To investigate lymphocyte proliferation when ADAR1 is suppressed in mixed lymphocyte culture test. METHODS: Primary lymphocyte was isolated from Balb/c and C57 mice. ADAR1 siRNA was transferred into the lymphocyte cell using Lipofectamine 2000 before twosource lymphocytes were mixed and cultured. The cell morphology was observed under a light microscope, the cell proliferation was checked using cell counting, trypan blue staining and the cell viability was determined by MTT assay. The ADAR1 mRNA was detected by RTPCR. RESULTS: When the ADAR1 expression was suppressed by the specific siRNA, the lymphocyte proliferation was inhibited in mixed lymphocyte culture test. CONCLUSION: ADAR1 has a potential role in the proliferation of mouse lymphocyte.
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