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人宫颈癌Ca Ski细胞IL
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作者：石华，成海恩，易发平，杨俊霞，张溢，马永平，宋方洲
【关键词】 白细胞介素24；克隆；真核表达；,Ca,Ski细胞
Molecular cloning, identification and eukaryotic expression of IL24 gene in Ca Ski cells
【Abstract】 AIM: To clone human IL24 (hIL24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL24 protein. METHODS: hIL24 gene was acquired by RTPCR from the total RNA of Ca Ski cell and cloned into plasmid pcDNA3.1(+) to generate the eukaryotic expression vector named pcDNA3.1(+)hIL24. pcDNA3.1(+)hIL24 was transfected into Ca Ski cells to express hIL24 protein. RESULTS: PCR and sequencing revealed that hIL24 gene was successfully cloned into plasmid pcDNA3.1(+) and its eukaryotic expression vector was constructed. The vector was able to express hIL24 protein after transfected into Ca Ski cells. CONCLUSION: The hIL24 eukaryotic expression vector has been successfully constructed.
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