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新生鼠脑缺血预处理后海马CA1区GAP
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【关键词】 脑缺血;缺血预处理；生长相关蛋白43；海马；Wistar大鼠；新生
Expression and significance of GAP43 in hippocampal CA1 region after cerebral ischemic preconditioning in neonatal rats
【Abstract】 AIM： To investigate the expression and significance of growthassociated protein43 (GAP43) in hippocampal CA1 region of 7dayold Wistar rats with cerebral ischemic preconditioning. METHODS： Cerebral ischemia in 7dayold Wistar rats was induced by occluding right common carotid artery for 45 min. The Wistar rats were randomly divided into shamoperation group (A), ischemiareperfusion group (B) and ischemic preconditioning group (C). In group C transient cerebral ischemia for 8 min was preformed before the serious cerebral ischemia (for 45 min) after 24 h interval. The pathological changes in the brain were observed by optical microscope, and immunohistochemistry and computer image analysis system were used to detect the expression of GAP43. RESULTS： No brain damage was found in group A， the neurocytes were degenerative and necrotic in group B. The pathological manifestations of the brain damage in group C were milder than those in the group B. The expression of GAP43 in hippocampal CA1 region in group B markedly increased at 24 h after reperfusion, reached peak at 7 d, and keep in a high level to 14 d after reperfusion (P<0.05 vs group A). The expression of GAP43 in hippocampal CA1 region of group C was higher compared with the group B and A; there were significant differences from 24 h to 14 d （P<0.05 vs group A and B）. CONCLUSION： The cerebral ischemic preconditioning can relieve the brain damage due to a subsequent more severe ischemia in neonatal rats. The expression of GAP43 markedly increased in hippocampal CA1 region in ischemic preconditioning group, which may be involved in the axonal plasticity and regeneration of neurocytes, one of the endogenous compensatory mechanisms of neuron after cerebral ischemia.
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