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以鼠尾胶为贴黏剂的小鼠淋巴管内皮细胞培养体系的建立
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作者：章必成，王俊，赵勇，高建飞，郭燕，陈正堂
【关键词】 鼠尾胶；贴黏剂；内皮,淋巴管；细胞培养
Establishment of culture system for mouse lymphatic endothelial cells adhered to rattail collagen
【Abstract】 AIM: To establish a convenient, cheap and stable culture system for mouse lymphatic endothelial cells (LEC). METHODS: Mouse lymphangiomas in abdominal cavity were induced by incomplete Freunds adjuvant, then disrupted and digested to obtain LEC, which were cultured in the flask or plate previously coated with rattail collagen. Expressions of LEC specific markers, VEGFR3 and LYVE1, were identified by immunofluorescence. The proliferation activity of LEC was evaluated by 3HTdR incorporation efficiency and cell doubling time. The capability of LEC to form lymphatic vessellike structures was assessed by the in vitro lymphatic vessel formation assay. RESULTS: Mouse lymphangiomas in abdominal cavity were induced successfully by incomplete Freunds adjuvant, and more than 98% living LEC were harvested. The expressions of VEGFR3 and LYVE1 were positive in LEC. In the rattail collagen coated flask or plate, LEC grew well, with 3HTdR incorporation efficiency increasing obviously and cell doubling time being (42.7±3.2) h. In the gel formed by rattail collagen, LEC could form lymphatic vessellike structures. CONCLUSION: Mouse LEC culture system using rattail collagen as adhesive is a convenient, cheap and stable culture system.
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