|
|
|
VAC对猪爆炸伤感染创面细菌数和G+/G-比例的影响
|
| |
作者：李望舟，李金清，李学拥，陈绍宗，李跃军，吕小星
【关键词】 爆震伤；伤口愈合；封闭负压引流技术；感染
Effect of vacuumassisted closure on bacteria load and G+ to G- bacteria ratio in pigs infected soft tissue blast injury
【Abstract】 AIM: To observe the effect of vacuumassisted closure (VAC) on the bacteria load and the ratio of G+ bacteria to G- bacteria in pigs infected soft tissue blast injury. METHODS: Sixteen blast wounds，created by explosion of a specific type of electric detonators which were fixed at 1 cm over the skin of the shoulders and hips of 4 small white domestic pigs (15-20) kg， were divided into 2 groups: the control group treated with conventional dressing change, and the experimental group treated with VAC at -15 kPa. All wounds were left untreated until the 3rd day after explosion and subsequently were infected. Tissue specimens, collected from the wounds before treatment and at different time points (1, 3, 6, 9, 14, and 19 d) after treatment, were weighed and homogenized, and each specimens homogenate, after diluted, was plated on blood agar and incubated at 37℃ for 24 h. After the number of bacterial colonies was counted, 200 out of the bacterial colonies of each specimen were processed for conventional Gramstaining and the rate of grampositive bacteria was calculated. RESULTS: Before treatment, the wound bacteria load of the 2 groups was up to about 3×107 cfu/g; it decreased to (1.91±0.245) ×105 cfu/g at day 3 after treatment in experimental group, and to (0.51±0.120) ×105 cfu/g at day 6; it decreased more slowly in control group to (19.54±3.67) ×105 cfu/g at day 9 after treatment and to (3.26±0.83) ×105 cfu/g at day 19 after treatment. Meanwhile, the rate of Grampositive bacteria was about 31%-36% of total bacteria in all wounds before treatment; that of experimental group increased to (55.58±2.98)% at day 6 after treatment and maintained at a high level thereafter; that of control group fluctuated between 30% and 40% from 1 to 19 d after injury. Compared with control group, experimental group had less bacteria and higher rate of grampositive bacteria (P<0.01) from 1 to 19 d after treatment. CONCLUSION: VAC can significantly decrease the bacteria load and the rate of Gramnegative bacteria in pigs infected soft tissue blast injury.
|
|
|
|
|
|
|
|
|
| 广告载入中... |
| 广告载入中... |
| 广告载入中... |
|
|
|
|
|
教育论文中心