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藜草花粉变应原免疫活性分析
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【西药学毕业论文】 作者：张洁，孙秀珍，陈曦，冯向莉，刘艳琴【关键词】 哮喘；藜草；花粉；变应原；变应原性；免疫原性Immunoactivity analysis of album pollen allergen
【Abstract】 AIM: To analyse protein components in album pollen and evaluate their allergenicity and immunogenicity. METHODS: The components of protein allergen were collected from the crude extract of chenopodium album pollen with Sephacryl S200 HR gel filtration for removing the impurities. Molecular weights of the components were tested through SDSPAGE. Then the reaction rates of IgE and IgG in asthmatic patients serum to chenopodium album pollen were counted by immunoblotting. RESULTS: Twelve bands of protein were obtained through SDSPAGE. The molecular weight of each band was 92, 63.1, 61, 52, 45, 43, 39.7, 38.9, 34, 31.6, 28.4 and 18.5~12 ku. Immunobloting identified 4 protein bands recognized by sIgE, molecular weights being 92, 34, 31.6 an
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