| | | 青蒿素对小鼠Lewis肺癌移植瘤生长和淋巴管生成的影响
| | 西医学 毕业小论文
作者：郭燕，王俊，章必成，陈正堂，高建飞，赵勇
【关键词】 青蒿素；癌,Lewis肺；淋巴管生成；血管内皮生长因子
Effect of artemisinin on metastatic tumor growth and lymphangiogenesis in mice bearing Lewis lung carcinoma
【Abstract】 AIM： To explore the effect of antimalarial artemisinin on Lewis lung carcimoma (LLC) growth and tumor lymphangiogenesis. METHODS： The models of LLC in C57BL/6 mice were established via subcutaneous injection of mouse LLC cells. After mice were orally administered with artemisinin at 50 mg/kg body weight, once 2 d for 2 weeks, tumors were measured with calipers twice weekly. Mice were sacrificed 30 d following tumor cell injection, and Lewis tumors and lungs were harvested and weighted. Lewis tumors and lungs were processed for immunohistochemical analysis for lymphangiogenesis using VEGFR3 and LYVE1 staining or for micrometastasis using HE staining. Lymphatic microvessel density (LMVD) was evaluated by LYVE1 positive lymphatic vessel count. Furthermore, mice were monitored for survival studies. RESULTS： LMVD, wet weight of lungs and number of lung metastasis in artemisinintreated group were significantly lower as compared with control group (P<0.05). However there was not statistically significant difference in tumor growth between artemisinintreated mice and control mice (P>0.05). In addition, artemisinintreated mice lived significantly longer than the control mice did (P<0.01). CONCLUSION： Antimalarial artemisinin effectively inhibits the lymphangiogenesis and lung metastasis, and enhances the survival of mice bearing LLC.
【Keywords】 artemisinin; carcinoma, Lewis lung; lymphangiogenesis; vascular endothelial grouth factorc
【摘要】 目的西医学 毕业小论文： 探讨抗疟药青蒿素对Lewis肺癌生长和淋巴管生成的西医学 毕业小论文作用. 方法： 通过接种（sc）Lewis肺癌细胞建立C57BL/6小鼠Lewis肺癌移植瘤模型，灌胃给予50 mg/kg的青蒿素，1次/2 d，连续给药2 wk；游标卡尺观察给药后肿瘤体积变化. 接种后第30 d处死动物，收集肿瘤组织和双肺组织，并称质量. 免疫组化检测瘤内和瘤周VEGFR3和LYVE1表达，计数LYVE1阳性淋巴管并计算微淋巴管密度，HE染色肺组织计数肺转移结节数，观察荷瘤小鼠的存活率. 结果： 与对照组比较，青蒿素组瘤内LMVD，肺湿重，肺转移发生率和肺转移结节数均较低（P<0.05）；青蒿素治疗组与对照组移植瘤生长没有明显差异（P>0.05）；青蒿素治疗组小鼠存活率与对照组比较明显增加（P<0.01）. 结论： 青蒿素可有效抑制Lewis肺癌淋巴管生成和肺转移，并提高小鼠存活率.
【关键词】 青蒿素；癌，Lewis肺；淋巴管生成；血管内皮生长因子C
0引言
淋巴结转移是多种肿瘤转移的重要途径，也是肿瘤预后的重要指标. 近年研究显示，肿瘤可以诱导淋巴管生成（lymphangiogenesis），并促进肿瘤细胞通过新生的淋巴管向区域淋巴结转移. 目前，多种淋巴管内皮特异性标记物的发现促进了人们对肿瘤淋巴管功能的认识，因此寻找可以抑制淋巴管生成的药物和方法已经成为抗肿瘤转移研究领域的热点［1］. 青蒿素（artemisinin）是1972年由我国科研人员从中药青蒿中分离得到的，具有抗疟作用的单体成分，现在临床上主要用于治疗耐药的恶性疟疾. 多项研究表明，青蒿素及其衍生物对多种肿瘤的生长具有抑制作用，并显示出较强的抗血管生成活性［2］. 然而，青蒿素是否可以抑制淋巴管生成还不清楚. 我们探讨了青蒿素对小鼠Lewis肺癌生长、淋巴管生成和肺转移的影响.
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