| | | 过钒酸钠对照射后BET
| | 临床医学毕业论文【关键词】 BET-2细胞；电离辐射；线粒体跨膜电位(ΔΨm)；细胞凋亡；过钒酸钠
摘要： 目的临床医学毕业论文 研究酪氨酸磷酸酶抑制剂过钒酸钠（Pervanadate，Per）对电离辐射作用后细胞线粒体膜电位(ΔΨm)改变和细胞凋亡的临床医学毕业论文影响。方法 应用流式细胞仪测定电离辐射作用后鼠源性髓系白血病细胞株BET-2细胞线粒体膜电位及其在过钒酸钠干预后的改变和细胞凋亡水平。结果 电离辐射早期可引起BET-2细胞线粒体膜电位降低，诱导细胞凋亡发生；过钒酸钠在一定程度上稳定细胞线粒体膜电位，减少细胞凋亡。结论 过钒酸钠可逆转电离辐射诱导的细胞线粒体膜电位的降低，减少造血细胞凋亡的发生。
关键词： BET-2细胞；电离辐射；线粒体跨膜电位(ΔΨm)；细胞凋亡；过钒酸钠
Effects of pervanadate on MMP of BET-2 cells induced by ionizing irradiation
Abstract： Objective To study the effects of pervanadate(Per),the inhibitor of protein tyrosine phosphotases on the alterations of mitochondrial membrane potential(MMP)and the apoptosis of BET-2 cells induced by ionizing irradiation.Methods BET-2 cells were divided into Per-treated groups and non-Per groups and incubated after ionizing irradiation.The MMP and apoptosis were measured with FCM.Results After irradiation was given,the MMP of BET-2 cells was reduced in the early stage significantly,and the apoptosis appeared,and these changes had a dose-dependent pattern and a time-course fashion.Per potently enhanced the MMP of BET-2 cells and prevented irradiation-treated cells from apoptosis.Conclusion Per reversed the repression of mitochondrial membrane potential and suppress apoptosis induced by ionizing irradiation in the hematopoietic cells.
Key words： BET-2 cell;ionizing irradiation;mitochondrial membrane potential(MMP,ΔΨm);apoptosis;pervanadate(Per)
细胞凋亡是电离辐射导致造血系统损伤的主要方式。预防与控制造血干/祖细胞大量凋亡，有效恢复机体造血功能，在未来可能爆发的核事故、核战争以及肿瘤放射治疗副反应控制等领域都具有十分重要的意义〔1〕。造血细胞信号转导过程受蛋白质酪氨酸磷酸酶（protein tyrosine phosphotases，PTP）的调控〔2〕。过钒酸钠（pervanadate，Per）作为PTP特异性抑制剂，参与细胞内多条信号通路信息的传递过程的调节〔3〕。通过改变PTP活性调控造血细胞受体信号转导通路，可能成为继重组人粒细胞集落刺激因子（G-CSF）、促红细胞生成素（EPO）、重组血小板生长因子（TPO）等细胞因子之外的促进辐射造血损伤修复的发展方向〔4〕。本研究观察Per对照射后BET-2细胞线粒体膜电位改变及细胞凋亡的影响，探讨调控造血细胞受体信号通路中PTP活性对造血细胞电离辐射损伤的保护作用。结果报告如下。
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