| | | 焦炉工人外周血淋巴细胞HSP 72表达特征
| | 西医学 免费论文下载【关键词】 焦炉逸散物；多环芳烃；苯并(a)芘；热休克蛋白72；影响因素,
摘要： 目的西医学 免费论文下载 研究焦炉工人外周血淋巴细胞热休克蛋白72(HSP72)表达特征及其影响因素。方法 选取某焦化厂焦炉工人267人和对照30人，蛋白印迹(Western blot)法检测外周血淋巴细胞HSP72的西医学 免费论文下载表达，高效液相色谱法测定作业环境空气中苯并(a)芘〔B(a)P〕浓度。收集研究对象个人信息。结果 炉顶工、炉侧工外周血淋巴细胞HSP72表达水平(G±SG)(120±047，130±037)显著高于对照组(085±034)，炉侧工显著高于炉底工(101±035)，差异有统计学意义(P<005)。不同作业环境〔B(a)P〕浓度与相应作业区工人外周血淋巴细胞HSP72表达水平呈二项式分布(R2=09999)。Logistic分析显示，与对照组相比，焦炉逸散物暴露引起淋巴细胞HSP72表达阳性的调整OR为131，其中炉顶工、炉侧工调整OR分别为149和269。多元线性回归分析显示，在低暴露水平下(炉底工、对照组)，吸烟指数和饮酒是HSP72表达水平升高的抑制因素(β分别为-00001和-005，P<005)。结论 焦炉逸散物可诱导外周血淋巴细胞HSP72表达升高，但高暴露剂量为焦炉逸散物对HSP72表达有抑制作用。低暴露水平下吸烟和饮酒对HSP72表达有抑制作用。
关键词：焦炉逸散物；多环芳烃；苯并(a)芘；热休克蛋白72；影响因素
Expressive characters of HSP72 in peripheral blood lymphocyte of coke oven workers
Abstract： Objective To investigate heat shock protein 72 (HSP72) levels in peripheral blood lymphocytes of coke oven workers and influence factors.Methods Two hundred sixtyseven coke oven workers and thirty controls without occupational PAHs exposure were investigated.Benzo(a)pyrene〔B(a)p〕concentrations in the ambient air were assayed by high performance liquid chromatography(HPLC).HSP72 levels in peripheral blood lymphocytes were measured by Westem Blot.Personal information was collected by questionnaire.Results HSP72 levels in oven-top,oven-side workers(120±047，130±037) were significantly higher than that in control(085±034,P<0.05),and HSP72 levels in oven-side workers were significantly higher than those in oven-bottom workers(101±035，P<005).〔B(a)p〕 concentrations and HSP72 levels in different exposed groups showed binomial distribution(R2=09999).The logistic regression analysis showed that the coke oven workers had significantly higher risk in HSP72 rising(adjust OR=131)than control,and the adjust OR of oven-top，oven-side workers were respectively 149 and 269.Multiple linear regression result presented that in the lower exposure group(oven-bottom workers and control),smoking and drinking were inhibiting factors to HSP72 expression(the regression coefficient were respectively -00001 and -005,P<005).Conclusion The coke oven emissions can induce the rising of HSP72 expression.But the high dose coke oven emissions showed inhibition to HSP72.Smoking and drinking played an inhibitional role in HSP72 expression in lower exposure group.
Key words： coke oven emission;polycyclic aromatic hydrocarbons;benzo(a)pyrene;heat shock protein 72;influence factor
热休克蛋白是生物体细胞在一系列应激因素作用下产生的具有高度保守性的一组细胞保护性蛋白质，在生理状态下，它们主要发挥细胞内功能〔1〕，其中热休克蛋白70 (heat shock protein 70,HSP70)家族与细胞保护之间的关系最密切，它在机体细胞耐受形成、机体免疫调节、遗传物质保护、基因调控、细胞增殖分化、肿瘤发生发展等过程中起重要作用〔2，3〕。HSP70家族主要有诱导型的HSP72和结构型的HSP73两个亚型〔4，5〕，在应激过程中特别是染毒过程中，主要为HSP72表达变化〔6〕。本研究旨在通过分析焦炉工人外周血淋巴细胞HSP72表达特征，探讨焦炉逸散物对HSP72表达的影响，为焦炉工人的健康监护和预防保护提供理论依据。
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