| | | 赛莱西布对肝癌HepG2细胞增殖与凋亡影响
| | 西医学 论文下载【关键词】 ,HepG2细胞;环氧合酶-2；赛莱西布；增殖;凋亡
摘要： 目的西医学 论文下载 探讨选择性环氧合酶-2(cox-2)抑制剂赛莱西布(celecoxib)对人肝癌细胞株HepG2细胞增殖、凋亡的西医学 论文下载影响及其可能的作用机制。方法 用不同浓度的赛来西布处理HepG2细胞，分别应用四甲基偶氮噻唑蓝试验、DNA梯度电泳法、放射免疫法检测赛亚西布对HepG2细胞增殖和凋亡及其cox-2活性的影响；应用免疫印迹法和比色法检测赛莱西布对HepG2细胞胱氨酸蛋白酶3(clasepase-3)蛋白质的表达及活性的影响。结果 125，25，50μmol/L的赛莱西布作用于HepG2细胞后，HepG2细胞增殖受抑制，与对照组比较，差异有统计学意义(P<001)；赛莱西布干预组HepG2细胞DNA明显降解，可见细胞凋亡特征性梯状DNA条带；干预组HepG2细胞caspase3蛋白表达及活性均明显升高，与对照组比较，差异均有统计学意义(P<001)；赛莱西布明显抑制HepG2细胞cox-2活性，与对照组比较，差异有统计学意义(P<001)。结论 赛莱西布可通过cox-2通路和caspase3通路抑制HepG2细胞增殖并诱导其凋亡。
关键词： HepG2细胞;环氧合酶-2；赛莱西布；增殖;凋亡
Effects of cyclooxygenase-2 on proliferation and apoptosis in HepG2 cells
Abstract： Objective To examine the effects of a selective inhibitor of cox-2,celecoxib on growth and apoptosis in HepG2 cells.Methods HepG2 cells were treated with various concentrations of celecoxib.Cell growth was measured by MTT,apoptosis was detected by DNA fragmentation assay,and cox-2 activity was measured by RIA;Expression of caspase-3 protein in HepG2 cells was determined by western blot,and caspase-3 activity was measured using colorimetric assay.Results After HepG2 cells were treated with different concentrations of celecoxib (12.5,25,50μmol/L),celecoxib could significantly suppress the growth of HepG2 cells respectively compared with the control group (P<0.01),and celecoxib-treated HepG2 cells produced a distinct oligosomal ladder,the characteristics of cells undergoing apoptosis;Meanwhile,The caspas-3 expression and activity of celecoxib treated HepG2 cells were increased respectively as compared with the control groups(P<0.01).Furthermore,the level of cox-2 activity was reduced in celecoxib-treated HepG2 cells respectively compared with the control group (P<0.01).Conclusion Celecoxib could inhibit the proliferation and increase apoptosis in HepG2 cells by cox-2 and caspase3 pathways.
Key words： HepG2 cell;cyclooxygenase-2;celeeoxib;proliferation;apoptosis
近年来的研究表明，环氧合酶-2(cox-2)在结直肠癌、食管癌、肺癌等多种人类肿瘤组织中高表达〔1-3〕。最新报道显示〔4，5〕，cox-2在肝癌组织中也高表达，并且在肝硬化组织中过表达，提示cox-2可能是肿瘤防治的一个新靶点〔3〕。非甾体类抗炎药(nonsteroidal anti-inflammatory drugs，NSAIDs)具有预防和辅助治疗肿瘤尤其胃肠道肿瘤的作用〔6〕，但NSAIDs抑制肿瘤的作用机制尚不明确。本研究选择NSAIDs类药物中的1种高选择性cox-2抑制剂赛莱西布(赛来西布)，作用于人肝癌细胞株HepG2观察其对HepG2细胞增殖与凋亡的影响，并探讨其可能的作用机制。结果报告如下。
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