| | | 内皮型一氧化氮合酶基因多态性与冠心病关系
| | 动物医学毕业论文【关键词】 ,冠心病；一氧化氮合酶,内皮；基因
摘要： 目的动物医学毕业论文 探讨内皮型一氧化氮合酶(eNOS)基因内含子4可变串联重复序列(VNTR)多态性与早发冠心病的动物医学毕业论文关系。方法 以医院为基础的病例对照研究，选择新诊断的冠心病患者为研究对象。男性≤55岁及女性≤65岁患冠心病为早发冠心病，以188例早发冠心病患者为病例组，315例迟发冠心病患者为对照组。应用聚合酶链反应(PCR)技术检测eNOS基因内含子4 VNTR多态性。结果 eNOS基因内含子4 VNTR c/c、c/b、b/b、b/a、a/a基因型频率，早发冠心病组分别为053%，000%，8138%，1649%，160%；迟发冠心病组分别是 000%，032%，7746%，2032%，190%。c/c+c/b+b/b与b/a+a/a基因型在2组之间分布差异无统计学意义(P=0268，OR=1294，95%CI=0820～2043)。c、b、a等位基因频率，早发冠心病组分别是053%，8963%，984%；迟发冠心病组分别是016%，8778%，1206%。c+b与a的等位基因频率在2组间分布差异无统计学意义(P=0280，OR=1257，95%CI=0830～1904)。在α=005显著性水平上，用多元逐步非条件Logistic回归分析调整性别、吸烟、饮酒、收缩压、体质指数、腰臀比、甘油三酯和总胆固醇后，eNOS基因内含子4 VNTR多态性与早发冠心病也无显著相关(P=0427, OR=0819, 95%CI=0500～1341)。结论 eNOS基因内含子4 VNTR多态性可能不是早发冠心病发病的危险因素。
关键词： 冠心病；一氧化氮合酶，内皮；基因
Association between polymorphism of endothelial nitric oxide synthase gene and coronary heart disease
Abstract： Objective To assess the association between intron 4 VNTR polymorphism in endothelial nitric oxide synthase (eNOS) gene and premature coronary heart disease(p-CHD).Methods A hospital-based case-control study was conducted.Newly-diagnosed CHD patients were recruited as this study subjects.one hurdred and eighty-eight CHD patients diagnosed at/before 55 years old for males and 65 for females were assigned to p-CHD case group with other 315 CHD patients as the control group.Genotypes were detected by polymorphism chain reaction (PCR) technique.Results The genotype frequencies of c/c,c/b,b/b,b/a,a/a were 053%,000%,8138%,1649%,160% in p-CHD group respectively and 000%,032%,7746%,2032%,190% in the control group respectively.There was no significant difference between the p-CHD and the control group for comparing genotype frequencies of c/c+c/b+b/b with b/a+a/a (P=0268,OR =1294,95%CI=0820～2043).The allele frequencies of c,b,a were 053%,8963%,984% in the p-CHD group respectively and 016%,8778%,1206% in the control group respectively.There was also no significant difference between the p-CHD and the control group for comparing allele frequencies of c+b with a (P=0280，OR=1257，95%CI=0830～1904).Stepwise multiple logistic regression analysis at 005 significant level with adjust ment of sex,smoking,alcohol drinking,systolic blood pressure,overweight,waist-hip ratio,serum triglyceride,serum total cholesterol covariates showed that the eNOS4a had no significant effect on p-CHD (P=0427,OR=0819,95%CI=0500～1341).Conclusion eNOS intron 4 a/b VNTR polymorphism is not a risk factor for p-CHD.
Key words： coronary heart disease;nitric oxide synthase;endothelial;gene
遗传因素在冠状动脉粥样硬化性心脏病(冠心病CHD)发病中具有重要作用，但早发与迟发病例在遗传背景方面存在较大差异，其中，内皮型一氧化氮合酶(eNOS)基因对冠心病具有重要作用〔1〕。eNOS基因内含子4存在27bp的可变串联重复序列(VNTR)，研究显示，这种多态性与机体一氧化氮(NO)的代谢水平密切相关〔2〕。为探讨eNOS基因内含子4VNTR多态性与早发冠心病之间的关系，本文对188例早发冠心病及315例迟发冠心病进行观察比较。结果报告如下。
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