| | | 不同剂量碘化钾对大鼠血液抗氧化能力影响
| | 中药学论文【关键词】 ,碘化钾；抗氧化能力；血液
摘要： 目的中药学论文 观察补充不同剂量碘化钾(KI)对Wistar大鼠血液抗氧化能力的中药学论文影响。方法 将Wistar大鼠随机分为6组：低碘组(LI)、适碘组(NI)、5倍高碘组(5HI)、10倍高碘组(10HI)、50倍高碘组(50HI)、100倍高碘组(100HI)。喂养3,6,12个月后，检测血液中的谷胱甘肽过氧化酶(GPx)活性、超氧化物歧化酶(SOD)活性以及丙二醛(MDA)含量。结果 LI组GPx活性在3个月和6个月时均低于NI组(P<001)，MDA含量在6个月时较NI组增高(P<005)。LI组SOD活性与NI组比较差异无统计学意义(P>005)。在实验3个月时，100HI组较NI组GPx活性降低(P<001)；在实验3个月、6个月和12个月时，4个高碘组SOD活性和MDA含量与NI组比较差异无统计学意义(P>005)。结论 低碘导致大鼠血液抗氧化能力降低；血液的抗氧化系统对高碘有一定的耐受能力；血液有较强的抗氧化能力和代偿能力。
关键词： 碘化钾；抗氧化能力；血液
Effect of different dosage potassium iodide on anti-oxidative capability of rats blood
Abstract： Objective To study the effect of different dosage KI on the anti-oxidative capability of blood.Methods The rats were divided into 6 groups and given by different dosage of KI,which were low iodide (LI),normal iodide (NI),5 fold high iodide (5HI),10 fold high iodide (10HI),50 fold high iodide (50HI) and 100 fold high iodide (100HI).Three,6 and 12 months later,the rats were sacrificed and blood glutathione peroxidase (GPx) activity,superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were measured.Results After treatment for 3 and 6 months,the GPx activity in LI group was markedly lower than that in NI group.Furthermore,after 6 months for low iodine intake,the MDA content in LI group was higher than that in NI group.The GPx activity in 100HI group was lower than that in NI group after administration for 3 months,but no difference between these two groups after treatment for 6 and 12 months,no difference was found in the GPx activity of NI group and those of 5HI,10HI and 50HI groups.There was no difference in SOD activity and MDA content among NI group and 4 high iodide groups.Conclusion Low iodide intake would damage the anti-oxidative capability of blood in normal rats.Blood had a strong anti-oxidative ability and compensative capabilities to compete with high iodide intake.
Key words： potassium iodide(KI);anti-oxidative capacity;blood
碘是合成甲状腺激素(TH)的必需微量元素，低碘或高碘都将导致甲状腺疾病。地方性甲状腺肿、Graves病、桥本氏病和甲状腺癌等甲状腺疾病发生和发展的重要机制之一是自由基(FR)的毒性作用〔1〕。有学者认为FR损伤作用也是碘导致的甲状腺疾病的发病机制之一〔2〕。为深入研究FR在碘致疾病中的作用，本实验观察了不同剂量碘化钾(KI)对血液抗氧化能力的影响。
1 材料与方法
11 实验动物与分组 选用断乳后1个月，体重120～140g的健康Wistar大鼠270只，随机分为6组：低碘组(LI组)、适碘组(NI组)、5倍高碘组(5HI组)、10倍高碘组(10HI组)、50倍高碘组(50HI组)、100倍高碘组(100HI组)，每组45只。喂养3，6，12个月后，股动脉取血处死大鼠。此外，12个月时LI组鼠死亡，故未取血测定。
12 实验方法 股动脉取血肝素抗凝后，采用南京建成生物工程研究所生产的生物试剂盒，按其说明测定抗凝全血谷胱甘肽过氧化酶(GPx)活性、红细胞超氧化物歧化酶(SOD)活性、血浆丙二醛(MDA)含量和血红蛋白(Hb)含量。
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