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结核分枝杆菌Rv2389基因真核表达质粒的构建及表达
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【关键词】 分枝杆菌,结核；Rv2389；基因表达
Construction and expression of eukaryotic expression vector of Mycobacterium tuberculosis Rv2389
【Abstract】AIM: To construct the eukaryotic expression vector encoding Mycobacterium tuberculosis Rv2389 and express it in CHO cells. METHODS: The gene encoding Rv2389 protein were amplified by polymerase chain reaction（PCR）from genome of Mycobacterium tuberculosis H37Rv strain. After sequenced, Rv2389 gene segments were subcloned into eukaryotic expression vector pCDNA3.1(-). The recombinant plasmid pCDNARv2389 were transfected into CHO cells with liposome. The expressions of mRNA and protein encoded by this gene were detected respectively with RTPCR and indirect immunofluoresent technology. RESULTS: Rv2389 was cloned into pCDNA3.1(-) correctly, and its expression at mRNA and protein levels was detected in CHO cells. CONCLUSION: Recombinant eukaryotic plasmid encoding Rv2389 was constructed successfully. The experiment established the basis for further study on the function of Rv2389.
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