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江苏地区不同类型乙型肝炎患者HBV基因分型研究
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【关键词】 肝炎病毒,乙型；基因型；聚合酶链反应
HBV genotyping in hepatitis B patients in Jiangsu province
【Abstract】 AIM: To investigate the distribution of hepatitis B virus (HBV) genotypes of hepatitis B patients in Jiangsu province and analyze the relationship of HBV genotypes with the serum HBV DNA levels of patients. METHODS: The HBV genotypes and HBV DNA levels of 176 patients in Jiangsu province were detected by realtime quantitative PCR and Taqman MGB probe technology.RESULTS: Among 176 HBVinfected patients, the proportions of genotype C, BC mixedtype, B, A and D were 66.5%(117/176), 22.7%(40/176), 5.1%(9/176), 0.6%(1/176) and 0.6%(1/176), respectively. The results showed there were significant differences between genotype C and genotype BC mixedtype in HBV DNA load(P<0.05), and the HBV DNA level of genotype BC mixedtype was significantly higher than that of genotype C. The rate of genotype C among the patients of chronic hepatitis B was 71.4%, higher than that among the patients of acute hepatitis B, liver cirrhosis and hepatocellular carcinoma, but it showed no significant differences in the various clinical types of HBV infection tested(P>0.05). CONCLUSION: Genotype C and genotype BC mixedtype are the two dominant genotypes in the patients in Jiangsu province, and genotype B, A, and D are also found, but not common. The HBV DNA load of genotype B, C mixedtype is significantly higher than that of genotype C.
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