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苦参碱对肝卵圆细胞增殖模型大鼠Notch1, Jagged1 mRNA表达的影响
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【关键词】 苦参碱；肝；卵母细胞；大鼠；Thy
Effect of matrine on expressions of Notch1, Jagged1 mRNA in hepatic oval cell proliferation model rats
【Abstract】 AIM: To study the expressions of Notch1, Jagged1 mRNA in hepatic oval cell proliferation model rats and the effect of matrine on them. METHODS: Fortyeight male SD rats were randomly separated into 4 groups according to body mass: model group(group A),lowdose matrine group( group B, 5 mg/100g), highdose matrine group (group C, 25 mg/100 g), control group (group D). The expression of Thy1 protein was detected by enzymehistochemisty, and the expressions of Notch1, Jagged1 mRNA were detected by semiquantitative RTPCR method. RESULTS: Compared with group D ，the expressions of Thy1 protein, Notch1 mRNA, Jagged1 mRNA in group A were singnificantly increased （P<0.01）. Compared with group A,the expressions of Thy1 protein, Notch1 mRNA,Jagged1 mRNA in group C were singnificantly increased（P<0.01）. CONCLUSION: Matrine could regulate Notch signaling, decrease the expression of Thy1 protein,which might be one of the mechanisms of inhibiting the proliferation of hepatic oval cells and inducing rat hepatic oval cells to differentiate into mature hepatocytes.
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