| | | 结直肠癌组织Fascin
| | 药学大专毕业论文【关键词】 Fascin
Expression of Fascin1 in human colorectal cancer and its significance
LIU Li, DING YanQing
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China
【Abstract】 AIM: To investigate the correlation between Fascin1 expression and carcinogenesis and biological behavior of colorectal cancer (CRC). METHODS: The expression of Fascin1 was examined in 80 specimens of CRC, 40 of paired normal colorectal mucosae and 37 of regional lymphatic metastases by immunohistochemical method. RESULTS: Fascin1 was expressed heterogeneously among the normal colorectal mucosae, CRC and lymphatic metastases (χ2＝30.917, P=0.000). Fascin1 was higher expressed in CRC compared with normal colorectal mucosae (Z=-3.912, P=0.000), and Fascin1 expression was significantly stronger in lymphatic metastases than that in primary CRC (Z＝-2.419, P=0.016). Furthermore, Fascin1 expression in CRC with lymph node metastasis was higher than that in CRC without lymph node metastasis (Z=-2.432, P＝0.015). Expression levels of Fascin1 protein, showing no correlation with the differentiation of CRC (χ2＝0.333, P=0.847), was positively correlated with the invasive depth of CRC (Z=-2.074, P=0.038). CONCLUSION: Fascin1 expression plays an important role in invasion and metastasis of CRC. Clinically it may be a useful indicator of the tumor invasion and metastasis in CRC.
【Keywords】 Fascin1; colorectal neoplesms; neoplasm invasiveness; lymphaticmetastasis
【摘要】 目的药学大专毕业论文： 探讨Fascin1蛋白表达与结直肠癌发生及其生物学行为的药学大专毕业论文关系. 方法： 应用免疫组化SP法，检测结直肠癌80例、正常黏膜40例以及结直肠癌区域淋巴结转移癌37例Fascin1蛋白的表达. 结果： 在结直肠正常黏膜、癌及淋巴结转移癌中Fascin1蛋白表达差异有显著性（χ2＝30.917, P=0.000）. 两两比较发现，癌组织Fascin1表达比正常黏膜强（Z=-3.912, P=0.000），淋巴结转移癌组织表达比原发结直肠癌组织强（Z＝-2.419, P=0.016）；在结直肠癌组织中，伴发淋巴结转移的结直肠癌组织比未发生转移的结直肠癌组织Fascin1表达明显增强，差异具有显著性（Z=-2.432, P＝0.015）；并且，Fascin1蛋白表达水平与结直肠癌的浸润深度呈正相关（Z=-2.074, P=0.038），而与结直肠癌的分化程度无关（χ2＝0.333, P=0.847）. 结论： Fascin1蛋白表达与结直肠癌的浸润、转移密切相关，可作为结直肠癌浸润、转移的指标.
【关键词】 Fascin1；结直肠肿瘤；肿瘤浸润；淋巴转移
0引言
Fascin1蛋白的主要功能是与肌动蛋白结合，并将其捆绑成平行的肌动蛋白束，使细胞形成线状或片足的突起，从而肿瘤细胞的运动性增加，侵袭转移能力增强［1］. Fascin1在人类的许多肿瘤中表达上调，如食道癌、胰腺癌等，并且与临床病理学和预后不良相关［2］. 目前Fascin1在人结直肠癌中的表达与临床病理的关系还不清楚，因此，我们采用免疫组化的方法检测正常结直肠黏膜、结直肠癌以及淋巴结转移癌组织中Fascin1表达情况，以探讨Fascin1表达与结直肠癌的发生及生物学行为的关系.
1材料和方法
1.1材料200201/200412手术切除结直肠癌标本80例，男52例，女28例，年龄40~75（平均55）. 正常结直肠黏膜40例取自手术切除标本边缘，区域淋巴结转移癌37例取自手术切除标本所附淋巴结. 全部标本术前均未接受化疗及放疗， 40 g/L中性甲醛固定，常规石蜡包埋，4 μm厚切片，分别进行HE染色和免疫组化检测.
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