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细胞外信号调节激酶在局灶性脑缺血中的表达
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【关键词】 脑缺血；再灌注；丝裂原激活蛋白激酶类；细胞凋亡；梗塞,大脑中动脉；大鼠
Expression of extracellular signalregulated kinase in focal cerebral ischemia
【Abstract】 AIM: To study the role of extracellular signalregulated kinase (ERK) during focal cerebral ischemiareperfusion in hippocampal neurons of rats. METHODS: Sixty male adult Wistar rats were randomly divided into 2 groups (n=30 per group): the sham operation group and the ischemiareperfusion group(I/R group). The rats of I/R group were subjected to 2 h of right middle cerebral artery occlusion (MCAO) with introducing a nylon suture to the right internal carotid artery and then 3, 6, 24, 48 and 72 h of reperfusion, respectively(as 5 subgroups, n=6 in each subgroup). The histopathologic changes were observed by HE staining. The apoptotic neurons were detected in CA1 area of hippocampus by TUNEL method. ERK phosphorylation was detected by immunohistochemistry. RESULTS: In I/R group as compared with shamoperation group, after MCAO, the survival neurons in CA1 areas decreased; a lot of apoptotic cells were observed and peaked at 48 h; ERK activity increased 3 h after MCAO and peaked at 6 h and returned to the normal level at 72 h. CONCLUSION: Cerebral ischemiareperfusion may result in the upregulation of ERK activity, and participate in the process of neuron apoptosis.
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