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增光片中五味子醇甲的含量测定
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[摘要] 目的 建立反相高效液相法测定增光片中五味子醇甲含量的方法。方法 采用十八烷基硅烷键合硅胶柱,流动相为甲醇-乙腈-水(60∶5∶32);检测波长为250nm。结果 五味子醇甲在0.0672~0.672μg范围内呈良好的线性关系;平均回收率为97.64%,RSD=1.21%。结论 该方法不仅操作简单、准确,且重复性好,可用于增光片的质量控制。 [关键词] 反相高效液相法;增光片;五味子醇甲;含量测定 Content determination of schizandrol A in Zengguang tablets [Abstract] Objective To study RP-HPLC methods that determined the schizandrol A content in Zengguang tablets.Methods Schizandrol A was separated completely on an C18 column,using a mixture of methanol,acetonitrile and water(60∶5∶32)as mobile phase,with UV detection at 250nm.Results Schizandrol A has better linear correlation between 0.0672μg and 0.672μg,the results also have shown the recovery rate was 97.64%,RSD 1.21%.Conclusion The methods is very stable and accurate,and has good reappearance.It can be used for quality control of Zengguang tablets.
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