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肺炎链球菌荚膜缺陷菌株的构建及功能研究
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【动物西医学论文】作者：孟江萍，尹一兵，袁军，张雪梅，蓝锴，陈淑惠，王虹，涂植光【Abstract】AIM：To construct galU-deletion mutant of streptococcus pneumoniae and get the capsule deficient strain, which can be used in pneumococcal functional genome research. METHODS: LFHPCR was introduced to generate a gene disruption construct consisting of Emr cassette with long flanking homology regions to the target gene. The streptococcus pneumoniae was then transformed directly with this PCR product. The galUdeletion mutant was obtained on the TSA agar containing erythromycin and identified by PCR. The deficient strain (5×106 CFU) was applied to infect BALB/c mice to determine the virulence. RESULTS: GalU gene was replaced completely by erm cassette. The mutant had low virulence to BALB/c mice. CONCLUSION: The galUdeletion mutant can be used to screen in vivopromoter in streptococcus pneumoniae because it is incapable of synthesizing
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