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枯草芽孢杆菌(Bacillus subtilis ZY-1)溶栓酶的分离纯化及其酶学性质
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【动物西医学论文】【摘要】 目的 分离纯化枯草芽孢杆菌(Bacillus subtilis ZY-1)溶栓酶,探讨该酶的理化性质。 方法 枯草芽孢杆菌培养,利用(NH4)2SO4沉淀、Sephadex G-100 柱层析对该酶发酵液进行分离纯化,利用四肽底物Sue-Ala-Ala-Pro-Phe-PNA作为底物, 研究 酶的理化性质。 结果 经发酵并纯化后获得的酶液其比活高达26 400 U/mg,酶反应最适温度45 ℃,最适pH 8.0,且具有较好的热稳定性。 结论 枯草芽孢杆菌溶栓酶具有溶血栓作用。
【关键词】 芽孢杆菌,枯草; 纤溶酶
ABSTRACT: Objective Purification of a fibrinolytic enzyme produced from Bacillus subtilis ZY-1 and investigation of its activity and characterization. Methods A high fibrinolytic enzyme was purified with (NH4)2SO4 fractionation and sephadex G-100 gelfiltration chromatography, and characterization was investigated by check the enzymatic activities in different conditions. Results The specific activity of enzyme was 26 400 U/mg, and its optimal temperature and pH was 45 ℃ and 8.0, respectively. The enzyme had fine thermal heat stability. Conclusion The enzyme had potential commercial value.
KEY WORD
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