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抗创伤弧菌mAb的制备与鉴定
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【##西医 论文】【摘要】 目的: 制备抗创伤弧菌mAb并用于创伤弧菌感染的快速诊断. 方法 : 用灭活创伤弧菌免疫Balb/c小鼠,采用杂交瘤技术制备抗创伤弧菌的mAb,ELISA法用于mAb的Ig效价,亚类及特异性鉴定. 结果: 获得了2株可持续分泌抗创伤弧菌mAb的杂交瘤细胞株. 结论: 获得的高特异性的抗创伤弧菌mAb不仅为创伤弧菌感染的快速诊断奠定了坚实的基础,而且为其进一步的 治疗 方面的 研究 提供了 理论 依据. 【关键词】 弧菌 创伤 抗体 单克隆 杂交瘤 【Abstract】AIM: To prepare monoclonal antibody (mAb) against Vibrio vulnificus and evaluate its roles in rapid diagnosis for Vibrio vulnificus infection. METHODS: Balb/c mice were immunized by inactived Vibrio vulnificus. Anti-Vibrio vulnificus McAbs were prepared by using hybridoma technique. The ELISA was used to identify Ig subgroup,titers of ascites,relative affinity and the specificity of McAbs. RESULTS: Two cell lines of hybridoma were established and they constantly secreted high quality McAbs steadily. CONCLUSION: The McAbs established could be highly specific for Vibrio vulnificus, which not only lays a solid foundation for de
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