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苯甲醇对红细胞冻干前海藻糖负载红细胞影响研究
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【西医 论文】【摘要】 为 研究 苯甲醇对海藻糖负载红细胞的 影响 ,在4℃条件下将红细胞孵育在浓度分别为10、30、50、100 mmol/L的苯甲醇海藻糖溶液中24小时,用氰化血红蛋白试剂盒测定海藻糖负载红细胞的溶血率,用硫酸蒽酮法检测红细胞内海藻糖浓度水平。结果表明:在100 mmol/L苯甲醇海藻糖溶液组,其红细胞内海藻糖浓度为72±12.98 mmol/L,与其它各组相比,有显著统计学差异(p=0.000);溶血率为17.99±3.75%,与其它各组相比,有显著统计学差异(p=0.000)。结论:苯甲醇可提高海藻糖负载红细胞的负载率,随着苯甲醇浓度的升高红细胞海藻糖负载率也提高,100 mmol/L的苯甲醇浓度为可用浓度。 【关键词】 苯甲醇 Effect of Benzyl Alcohol on Trehaloseloading Red Blood Cells before Lyophilization Abstract This study was aimed to evaluate the effect of benzyl alcohol on trehaloseloading red blood cells (RBCs). The RBCs were incubated in 10, 30, 50 and 100 mmol/L concentrations of benzyl alcoholtrehaloe solution at 4℃ for 24 hours. The hemolysis rate of loaded RBCs was detected by using cyanohemoglobin kit, the intracellular trehalose level were assayed by sulfate anthrone method. The results showed that the intracellular trehalose concentration in
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